Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage
Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage
复制标题
DOI：
10.1128/mcb.01118-06
复制
发表时间：
2006-12-01
影响因子：
5.3
通讯作者：
Friedberg, Errol C.
中科院分区：
文献类型：
--
作者：
Guo, Caixia;Tang, Tie-Shan;Friedberg, Errol C.
Rev1蛋白是DNA聚合酶Y家族中的一个真核成员，参与复制旁路对DNA损伤的耐受。Rev1在这一过程中的确切作用(S)尚不清楚。在这里，我们表明，通过酵母双杂交试验和谷胱甘肽S-转移酶下拉试验，小鼠REV1可以物理上与泛素相互作用。Rev1与泛素的结合需要位于Rev1 C末端的泛素结合基序(UBM)。UBMS还介导了单一泛素化的增殖细胞核抗原和Rev1之间的增强关联。在暴露于紫外线辐射的细胞中，Rev1与复制焦点的关联依赖于功能上的UBM。在体内，Rev1的UBM被证明有助于DNA损伤耐受和损伤诱导的突变。
REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of REV1 with ubiquitin requires the ubiquitin-binding motifs (UBMs) located at the C terminus of REV1. The UBMs also mediate the enhanced association between monoubiquitylated PCNA and REV1. In cells exposed to UV radiation, the association of REV1 with replication foci is dependent on functional UBMs. The UBMs of REV1 are shown to contribute to DNA damage tolerance and damage-induced mutagenesis in vivo.