A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior
A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior
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DOI：
10.1016/j.neuron.2015.03.027
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发表时间：
2015-05-06
期刊：
影响因子：
16.2
通讯作者：
Svoboda, Karel
中科院分区：
文献类型：
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作者：
Peron, Simon P.;Freeman, Jeremy;Svoboda, Karel
由于每个大脑区域都有大量的神经元，因此全面测量神经活动仍然具有挑战性。我们在表达GCaMP 6s和核红色荧光蛋白的小鼠中使用体积双光子成像，以在相关皮质柱中对75%的浅桶皮质神经元的活性进行采样，每只动物约12，000个神经元，在执行单个胡须物体定位任务期间。任务相关的活动达到高峰期间对象触诊。编码模型将活动与行为变量联系起来。在对应于备用胡须的列中，300个层（L）2/3锥体神经元（17%）各自编码触摸和胡须运动。触摸表示下降了一半，周围列，胡须运动表示不变。随着刻板的任务相关运动的出现，感觉表征没有表现出可测量的可塑性。触摸方向是地形组织的，具有被动和主动触摸的不同组织。我们的工作揭示了多个触觉特征的稀疏和空间混合表示。
Comprehensive measurement of neural activity remains challenging due to the large numbers of neurons in each brain area. We used volumetric two-photon imaging in mice expressing GCaMP6s and nuclear red fluorescent proteins to sample activity in 75% of superficial barrel cortex neurons across the relevant cortical columns, approximately 12,000 neurons per animal, during performance of a single whisker object localization task. Task-related activity peaked during object palpation. An encoding model related activity to behavioral variables. In the column corresponding to the spared whisker, 300 layer (L) 2/3 pyramidal neurons (17%) each encoded touch and whisker movements. Touch representation declined by half in surrounding columns; whisker movement representation was unchanged. Following the emergence of stereotyped task-related movement, sensory representations showed no measurable plasticity. Touch direction was topographically organized, with distinct organization for passive and active touch. Our work reveals sparse and spatially intermingled representations of multiple tactile features.