Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment
Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment
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DOI：
10.1016/j.clinbiochem.2014.03.019
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Baranczyk-Kuzma, Anna
中科院分区：
文献类型：
--
作者：
Chrzanowska, Alicja;Grabon, Wojciech;Baranczyk-Kuzma, Anna
目的：探讨精氨酸酶活性及其表达在肝病诊断中的价值。设计与方法：检测140例患者血清精氨酸酶活性、敏感性和特异性，其中肝细胞癌50例，肝细胞癌60例，胆总管结石30例，正常对照组90例。观察肿瘤切除或肝移植前后肝细胞癌和肝细胞癌患者血清精氨酸酶活性。比较精氨酸酶与甲胎蛋白(AFP)和转氨酶(AST、ALT)的敏感性。在LC组，移植前后的胆汁中也检测了该酶的活性。结果：肝细胞癌和肝细胞癌患者术前血清精氨酸酶活性明显高于对照组，术后明显下降。精氨酸酶在肝癌中的敏感性显著高于AFP、AST和ALT(分别为96%、40%、20%和18%)。肝细胞癌高于LC(93%)和CDL(33%)。精氨酸酶的特异性在80%以上。在肝硬变患者的胆汁中，活性最高的是肝移植后即刻。随着时间的延长而降低，但在移植排斥时急剧增加。结论：血清精氨酸酶活性的升高与同工酶AII的存在对肝癌和LC的诊断有一定的参考价值。胆汁中精氨酸酶活性的测定可能有助于监测肝移植受者。(C)2014年加拿大临床化学家学会。爱思唯尔公司出版，版权所有。
Objective: To assess the utility of arginase activity and expression in diagnosis of liver diseases.Design and methods: Arginase activity, sensitivity and specificity were determined in serum of 140 patients including 50 with HCC, 60 with LC, 30 with choledocholithiasis (CDL) and 90 healthy controls. In HCC and LC arginase activity in serum was studied before and after tumor resection or liver transplantation. Arginase sensitivity in HCC was compared to that of alpha-fetoprotein (AFP) and aminotransferases (AST, ALT). In LC the activity was determined also in bile before and after transplantation. The expression of arginase isoenzymes in serum was studied by Western blotting.Results: In HCC and LC the preoperative arginase activity was significantly higher compared to controls, and it decreased after surgery. The sensitivity of arginase in HCC was much higher than that of AFP, AST and ALT (96, 40, 20 and 18%, respectively). In HCC it was higher than in LC (93%) and CDL (33%). The specificity of arginase was above 80%. In bile of cirrhotic patients the highest activity was immediately after liver transplantation. It decreased with time but increased dramatically at the time of the graft rejection. Arginase AII was present in serum of HCC and LC but not the control cases.Conclusions: The increase of arginase activity in serum accompanied by the presence of isoenzyme AII can be useful in HCC and LC diagnosis. The determination of arginase activity in bile may be helpful in monitoring liver graft recipients. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.