In vivo bronchodilator activity of vasoactive intestinal peptide in the cat.
In vivo bronchodilator activity of vasoactive intestinal peptide in the cat.
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猫体内血管活性肠肽的体内支气管扩张活性。
DOI：
10.1164/arrd.1983.128.5.827
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Altiere,RJ
中科院分区：
文献类型：
--
作者：
Diamond,L;Szarek,JL;Gillespie,MN;Altiere,RJ
血管活性肠肽（VIP）是一种广泛存在于动物界的八肽，分布于多种器官和组织中，具有广泛的生物学作用。我们评估了VIP在麻醉、机械通气、阿托品化、闭胸猫中产生支气管扩张的能力。静脉推注VIP（0.1至10µ g·kg-1）以剂量依赖性方式逆转了静脉输注5-羟色胺（5-HT）（5至30µg·kg-1·min-1）诱导的肺阻力（RL）增加和动态肺顺应性（Cdyn）降低。通过使用RL作为中心气道口径的指数和动态弹性（Edyn; Cdyn的倒数）作为外周气道和肺实质的张力的指数来评估支气管扩张剂活性的分布。虽然各级气管支气管树响应VIP，主要的作用部位似乎是在中央气道。前列腺素E2（PGE 2）（0.1 - 10µg·kg-1）静脉给药后，观察到类似的松弛作用分布和幅度。超声雾化吸入前列腺素E2也能有效地逆转5-HT引起的支气管收缩，而VIP则不能。VIP介导的支气管扩张作用在吲哚美辛或普萘洛尔预处理后持续存在。我们的结论是VIP是一种有效的松弛剂的猫气道平滑肌在vivoand这种效果的肽发生独立的前列腺素生产或β肾上腺素能受体激活。
Vasoactive intestinal peptide (VIP) is an octacosapeptide that occurs widely in the animal kingdom, is distributed in many organs and tissues, and has a broad range of biologic actions. We evaluated the ability of VIP to produce bronchodilation in anesthetized, mechanically ventilated, atropinized, closed chest cats. Boluses of VIP injected intravenously (0.1 to 10µg-kg-1) reversed in a dose-dependent manner the increase in lung resistance (RL) and the decrease in dynamic lung compliance (Cdyn) induced by an intravenously administered infusion of 5-hydroxytryptamine (5HT) (5 to 30µg·kg-1·-min-1). Distribution of bronchodilator activity was assessed by using RL as an index of central airways caliber and dynamic elastance (Edyn; the inverse of Cdyn) as an index of tone in peripheral airways and lung parenchyma. Although all levels of the tracheobronchial tree responded to VIP, the predominant site of action appeared to be in central airways. A similar distribution and magnitude of relaxant effects were observed after the intravenous administration of prostaglandin E2(PGE2) (0.1 to 10µg·kg-1). Prostaglandin E2also was effective in reversing 5HT-induced bronchoconstriction when given by ultrasonic nebulization, whereas VIP was not. Bronchodilation mediated by VIP persisted after pretreatment with indomethacin or propranolol. We conclude that VIP is a potent relaxant of feline airways smooth musclein vivoand that this effect of the peptide occurs independent of prostaglandin production or beta adrenergic receptor activation.