GC level and expression of human coding sequences
GC level and expression of human coding sequences
复制标题
DOI：
10.1016/j.bbrc.2007.12.155
复制
发表时间：
2008-03-14
影响因子：
3.1
通讯作者：
Bernardi, Giorgio
中科院分区：
文献类型：
--
作者：
Arhondakis, Stiliano's;Clay, Oliver;Bernardi, Giorgio
几个小组已经解决了GC对哺乳动物基因表达水平影响的问题。一般来说. .富含gc的基因似乎比缺乏gc的基因表达得更多。最近，为了消除与基因组背景相关的差异，研究人员比较了插入载体质粒中的GC(3)-富基因和GC(3)-差基因（GC(3)是第三个密码子位置GC）的表达水平。转染实验表明，GC(3)富集基因的表达效率高于GC(3)缺失基因的表达效率，表明GC3在本质上显著提高了GC(3)的表达效率。在这里，我们表明，虽然使用的协议消除了原始的基因组背景，但他们用质粒背景取代了它，其组成特性影响了结果。(C) 2007爱思唯尔公司版权所有。
Several groups have addressed the issue of the influence of GC on expression levels in mammalian genes. In general.. GC-rich genes appeared to be more expressed than GC-poor ones. Recently, expression levels of GC(3)-rich and GC(3)-poor versions of genes (GC(3) is the third codon position GC), inserted in vector plasmids, were compared in order to eliminate differences associated with their genomic context. Transfection experiments showed that GC(3)-rich genes were expressed more efficiently than their GC(3)-poor counterparts, indicating that GC3 dramatically and intrinsically boosts expression efficiency. Here we show that, while the protocols used eliminated the original genomic context, they replaced it with the plasmid contexts whose compositional properties affected the results. (C) 2007 Elsevier Inc. All rights reserved.