Actin-based metallic nanowires as bio-nanotransporters
Actin-based metallic nanowires as bio-nanotransporters
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DOI：
10.1038/nmat1205
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发表时间：
2004-10-01
期刊：
影响因子：
41.2
通讯作者：
Willner, I
中科院分区：
文献类型：
--
作者：
Patolsky, F;Weizmann, Y;Willner, I
导电纳米线或图案化导电纳米元件的合成是未来纳米级电路制造的一个具有挑战性的目标(1)。同样，纳米尺度力学的实现可能会为纳米生物技术领域带来一个新的方面。在这里，我们报道了导电和图案化的肌动蛋白基金纳米线的设计，以及三磷酸腺苷驱动纳米物体的运动性。用金纳米粒子标记G-肌动蛋白的聚合，然后催化放大纳米粒子，得到长1-4微米，高80-200 nm的金线，具有高的导电性。Au纳米粒子/G-肌动蛋白单体聚合后，游离G-肌动蛋白聚合，或者金纳米粒子标记的G-肌动蛋白在聚合的F-肌动蛋白上聚合，然后颗粒催化放大，分别得到图案化的肌动蛋白-金线-肌动蛋白或金线-肌动蛋白-金线纳米结构。我们展示了肌球蛋白界面上肌动蛋白-Au丝-肌动蛋白细丝在三磷酸腺苷(ATP)作用下的运动性。这些基于肌动蛋白的金属丝及其纳米传输功能为开发生物/无机杂化装置引入了新的概念。
The synthesis of conductive nanowires or patterned conductive nanoelements is a challenging goal for the future fabrication of nanoscale circuitry(1). Similarly, the realization of nanoscale mechanics might introduce a new facet to the area of nanobiotechnology. Here we report on the design of conductive and patterned actin-based gold nanowires, and on the ATP-driven motility of the nano-objects. The polymerization of G-actin labelled with Au nanoparticles, followed by the catalytic enlargement of the nanoparticles, yields gold wires (1-4 mum long and 80-200 nm high) exhibiting high electrical conductivity. The polymerization of the Au nanoparticle/G-actin monomer followed by the polymerization of free G-actin, or alternatively the polymerization of the Au-nanoparticle-labelled G-actin on polymerized F-actin followed by the catalytic enlargement of the particles, yields patterned actin-Au wire-actin or Au wire-actin-Au wire nanostructures, respectively. We demonstrate the ATP-fuelled motility of the actin-Au wire-actin filaments on a myosin interface. These actin-based metallic wires and their nanotransporting funcionality introduce new concepts for developing biological/inorganic hybrid devices.