Synthesis, processing, and subcellular localization of mouse ADAM3 during spermatogenesis and epididymal sperm transport
Synthesis, processing, and subcellular localization of mouse ADAM3 during spermatogenesis and epididymal sperm transport
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DOI：
10.1262/jrd.50.571
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发表时间：
2004-10-01
影响因子：
1.8
通讯作者：
Baba, T
中科院分区：
文献类型：
--
作者：
Kim, E;Nishimura, H;Baba, T
为了阐明小鼠ADAM 3（cyritestin）在精子发生和附睾精子运输过程中的合成、加工和亚细胞定位，我们使用亲和纯化的抗ADAM 3抗体对睾丸生殖细胞、附睾和输精管精子进行了免疫印迹和免疫组化分析。ADAM 3最初在圆形精子细胞中合成为110-kDa的前体，然后在精子运输到附睾中和/或在附睾中一次的过程中，前体被加工成42-kDa的成熟蛋白。成熟的ADAM 3定位于获能精子头部的前部，并在钙离子载体A23187诱导的顶体反应中迅速从头部区域去除。这些结果表明，ADAM 3的成熟形式参与精子与卵子透明带的结合，而不是精子与卵子之间的膜融合。
To elucidate synthesis, processing, and subcellular localization of mouse ADAM3 (cyritestin) during spermatogenesis and epididymal sperm transport, we carried out immunoblotting and immunohistochemical analysis of testicular germ cells, and epididymal and vas deferens sperm, using affinity-purified anti-ADAM3 antibody. ADAM3 was initially synthesized as a 110-kDa precursor in round spermatids, and the precursor was then processed into a 42-kDa mature protein during the sperm transport into and/or once in the epididymis. The mature ADAM3 was localized on the anterior part of capacitated sperm heads and was rapidly removed from the head region during the calcium ionophore A23187-induced acrosome reaction. These results demonstrate that the mature form of ADAM3 is involved in the binding of sperm to the egg zona pellucida, not in the membrane fusion between sperm and egg.