Induction of Hsp27 and Hsp32 stress proteins and vimentin in glial cells of the rat hippocampus following hyperthermia
Induction of Hsp27 and Hsp32 stress proteins and vimentin in glial cells of the rat hippocampus following hyperthermia
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DOI：
10.1023/a:1024268126310
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发表时间：
2003-08-01
影响因子：
4.4
通讯作者：
Brown, IR
中科院分区：
文献类型：
--
作者：
Bechtold, DA;Brown, IR
在应激刺激下，细胞通过诱导一系列热休克蛋白（hsps）来应答，这些蛋白在修复和保护机制中发挥重要作用。本研究探讨热休克蛋白27和热休克蛋白32在成年大鼠海马的表达模式，全身高温。这些低分子量应激蛋白的显著诱导在使用细胞特异性标志物（星形胶质细胞的GFAP和小胶质细胞的凝集素GSA I-B4）鉴定的胶质细胞群体中是明显的，所述胶质细胞群体例如星形胶质细胞和小胶质细胞。高温也导致了一个强大的诱导中间丝蛋白，波形蛋白，在成年大鼠海马神经胶质细胞。有趣的是，在微血管系统中观察到Hsp 27和波形蛋白的快速诱导，表明高温应激可能损害血脑屏障。
In response to stressful stimuli, cells respond by inducing a set of heat shock ( stress) proteins ( hsps) that play important roles in repair and protective mechanisms. The present study investigates the expression patterns of Hsp27 and Hsp32 in the adult rat hippocampus following whole body hyperthermia. A pronounced induction of these low-molecular-weight stress proteins was apparent in populations of glial cells such as astrocytes and microglia that were identified using cell-specific markers (GFAP for astrocytes and the lectin GSA I-B4 for microglia). Hyperthermia also resulted in a robust induction of the intermediate filament protein, vimentin, in glial cells in the adult rat hippocampus. Interestingly, a rapid induction of both Hsp27 and vimentin was observed in the microvasculature, suggesting that hyperthermic stress may compromise the blood-brain barrier.