The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells.
The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells.
复制标题
生慧汤方促进PC12细胞神经突生长和GAP-43表达水平
DOI：
10.1186/s12906-016-1339-y
复制
发表时间：
2016-09-20
影响因子：
--
通讯作者：
Hua Q
中科院分区：
文献类型：
--
作者：
Zhang Q;Zhang ZJ;Wang XH;Ma J;Song YH;Liang M;Lin SX;Zhao J;Zhang AZ;Li F;Hua Q
生慧汤是临床上用于治疗健忘症的传统中草药。为了解益气健脾方药的组方原理，观察益气健脾方药组（益气健脾方药组）中茯苓（Schw.）狼人参组和由远志组成的“化痰益智”组，菖蒲和白芥子对PC 12细胞突起生长的影响，探讨了两个中药方剂的拆方生灰汤及其作用的分子机制。首先，采用CCK-8试剂盒检测两种药物对PC 12细胞活力的影响;采用流式细胞仪检测两种药物作用后PC 12细胞凋亡的情况。其次，观察两方对PC 12细胞分化的影响。RT-PCR和western blot检测GAP-43 mRNA和蛋白表达水平。益气健脾方呈剂量依赖性地降低细胞活力，但对细胞凋亡无明显影响。同时，促进神经突起生长，提高GAP-43 mRNA和蛋白表达水平。化痰益智方对细胞活力和凋亡也有类似的影响。此外，它还可以促进细胞突起的生长，GAP-43 mRNA和蛋白的表达水平较高。生慧汤益气健脾、化痰益智方药对神经突起生长有促进作用。其作用与上调GAP-43的表达有关。本文的在线版本（doi：10.1186/s12906-016-1339-y）包含补充材料，可供授权用户使用。
Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated. Firstly, CCK-8 kit was used to detect the impact of the two prescriptions on PC12 cell viability; and Flow cytometry was performed to measure the cell apoptosis when PC12 cells were treated with these drugs. Secondly, the effect of the two prescriptions on the differentiation of PC12 cells was observed. Finally, the mRNA and protein expression levels of GAP-43 were analyzed by RT-PCR and western blot, respectively. “Tonifying qi and strengthening spleen” prescription decreased cell viability in a dose-dependent manner, but had no significant effect on cell apoptosis. Meanwhile, it could improve neurite growth and elevate the mRNA and protein expression level of GAP-43. “Eliminating phlegm and strengthening intelligence” prescription also exerted the similar effects on cell viability and apoptosis. Furthermore, it could also enhance cell neurite growth, with a higher expression level of GAP-43 mRNA and protein. “Tonifying qi and strengthening spleen” and “eliminating phlegm and strengthening intelligence” prescriptions from Shenghui soup have a positive effect on neurite growth. Their effects are related to the up-regulating expression of GAP-43. The online version of this article (doi:10.1186/s12906-016-1339-y) contains supplementary material, which is available to authorized users.
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DOI：
10.1196/annals.1377.013
发表时间：
2006-01-01
期刊：
INTEGRATED MOLECULAR MEDICINE FOR NEURONAL AND NEOPLASTIC DISORDERS
影响因子：
--
作者：
Yoshimura, Takeshi;Arimura, Nariko;Kaibuchi, Kozo
通讯作者：
Kaibuchi, Kozo
DOI：
10.1016/j.mcn.2014.02.002
发表时间：
2014-03
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Lerch JK;Martínez-Ondaro YR;Bixby JL;Lemmon VP
通讯作者：
Lemmon VP
影响因子：
3.7
作者：
Bonds JA;Kuttner-Hirshler Y;Bartolotti N;Tobin MK;Pizzi M;Marr R;Lazarov O
通讯作者：
Lazarov O
影响因子：
64.5
作者：
AIGNER, L;ARBER, S;CARONI, P
通讯作者：
CARONI, P
影响因子：
5.6
作者：
Jose Esposito, Maria;Occhionero, Miranda;Cicogna, PierCarla
通讯作者：
Cicogna, PierCarla