Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next-generation sequencing
Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next-generation sequencing
复制标题
通过新一代测序评估增殖性糖尿病视网膜病变房水中 microRNA 的分布情况
DOI：
10.1111/ceo.13554
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发表时间：
2019-09-01
影响因子：
4
通讯作者：
Lu, Lin
中科院分区：
文献类型：
--
作者：
Chen, Shida;Yuan, Miner;Lu, Lin
研究背景microRNA（miRNA）在糖尿病视网膜病变的发病机制中起重要作用。我们尝试使用下一代测序（NGS）探索增殖性糖尿病视网膜病变（PDR）房水中的miRNA和piwi相互作用RNA（皮尔纳）谱。方法收集9只PDR眼和9只白内障对照眼的房水样本，进行NGS。使用定量聚合酶链反应（qPCR）来验证测序结果。氧诱导视网膜病变（OIR）模型用于验证血管生成相关的miRNA。结果共检测到484条miRNAs在PDR眼和白内障对照眼中的差异表达，其中成熟miRNAs 210条，新发现miRNAs 274条。此外，8种miRNAs和30种piRNAs被鉴定为两组之间表达差异最大的（P > 0.85）。这种差异表达的miRNA被预测为调节Rho蛋白信号转导、神经递质摄取和组蛋白赖氨酸甲基化。通过qPCR确认miR-184、-150-5p和-93-5p的相对表达模式。在OIR模型中证实了miR-93- 5 p的表达减少。结论PDR患者眼房水中miRNA和皮尔纳的表达水平与PDR的发病机制密切相关，miRNA和piRNA可能作为PDR的潜在生物标志物。
Background The microRNAs (miRNA) have been found to play an important role in the pathogenesis of diabetic retinopathy. We try to explore the miRNA and piwi-interacting RNA (piRNA) profile in the aqueous humour of proliferative diabetic retinopathy (PDR) using next-generation sequencing (NGS). Methods Aqueous humour samples were collected from nine PDR eyes and nine cataract control eyes, and NGS was performed. Quantitative polymerase chain reaction (qPCR) was used to validate the sequencing results. An oxygen-induced retinopathy (OIR) model was used to validate the angiogenesis related miRNA. Results In total, 484 miRNAs were differently expressed between the PDR eyes and cataract control eyes, including 210 mature miRNAs and 274 novel miRNAs. Furthermore, eight miRNAs and 30 piRNAs were identified as the most differently expressed between the two groups (P > .85). This differential expression of miRNA was predicted to regulate Rho protein signal transduction, neurotransmitter uptake and histone lysine methylation. Relative expression patterns of miR-184, -150-5p and -93-5p were confirmed by qPCR. A reduced expression of miR-93-5p was confirmed in the OIR model. Conclusions This study comprehensively demonstrated the miRNA and piRNA expression profile of the aqueous humour of PDR eyes, which may serve as a potential biomarker and involved in the pathogenesis of PDR.