Evaluation of Pluripotency in Human Dental Pulp Cells
Evaluation of Pluripotency in Human Dental Pulp Cells
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DOI：
10.1016/j.joms.2008.09.011
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发表时间：
2009-03-01
影响因子：
1.9
通讯作者：
Bessho, Kazuhisa
中科院分区：
文献类型：
--
作者：
Koyam, Noriaki;Okubo, Yasunori;Bessho, Kazuhisa
目的：出生后干细胞已从各种组织中分离，包括骨髓、神经组织、皮肤、视网膜和牙上皮。最近，已经从人牙髓中分离出成体干细胞。出生后的干细胞已经从各种组织中分离出来。以往普遍认为，生后干细胞的分化潜能受到谱系的限制。材料与方法：收集成年人正常阻生第三磨牙和正常脱落乳牙（SHED;来自人类脱落乳牙的干细胞）。结果：BMP-2处理组在细胞中产生碱性磷酸酶，并且还在培养基中产生和分泌骨钙素，并且能够诱导骨钙素或Sox 9，Col 2，逆转录聚合酶链反应（RT-PCR）检测Col X的表达。对于成脂分化，有潜力的SHED和牙髓干细胞（DPSC）表达2脂肪细胞特异性转录，过氧化物酶体增殖物激活受体γ 2和LPL，在体外，做骨髓间充质干细胞通过RT-PCR.Conclusion：这项研究表明，多能细胞分离的人牙髓在体外扩增和分化成骨细胞，软骨细胞和脂肪细胞。DPSC和SHED不仅来源于非常容易获得的组织资源，而且能够为潜在的临床应用提供足够的细胞。(C)2009年美国口腔颌面外科医师协会
Purpose: Postnatal stem cells have been isolated from various tissues, including bone marrow, neural tissue, skin, reina, and dental epithelium. Recently, adult stem cells have been isolated from human dental pulp. Postnatal stem cells have been isolated from a variety of tissues. Previously, it was generally accepted that the differentiation potential of postnatal stem cells was lineage restricted.Materials and Methods: Normal impacted third molars were collected from adults and normal exfoliated deciduous teeth (SHED; stem cells from human exfoliated deciduous teeth) by single-colony selection and magnetic activated cell sorting.Results: BMP-2 treatment groups produced alkaline phosphatase in the cells and also produced and secreted osteocalcin in the culture medium, and were capable of inducing an upregulated expression of Osteocalcin or Sox9, Col 2, and Col X by reverse transcriptase polymerase chain reaction (RT-PCR). For adipogenic differentiation, there is potential for SHED and dental pulp stem cells (DPSC) to express 2 adipocyte-specific transcripts, PPAR gamma 2 and LPL, in vitro, as do bone marrow mesenchymal stem cells by RT-PCR.Conclusion: This study demonstrated that pluripotential cells isolated from the pulp of human teeth expanded in vitro and differentiated into osteoblasts, chondrocytes, and adipocytes. DPSC and SHED are not only derived from a very accessible tissue resource but also capable of providing enough cells for potential clinical applications. (C) 2009 American Association of Oral and Maxillofacial Surgeons