Autoimmune diseases linked to abnormal K+ channel expression in double-negative CD4-CD8- T cells.
Autoimmune diseases linked to abnormal K+ channel expression in double-negative CD4-CD8- T cells.
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自身免疫性疾病与双阴性 CD4-CD8-T 细胞中 K 通道表达异常有关。
DOI：
10.1002/eji.1830200406
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发表时间：
1990
影响因子：
5.4
通讯作者：
Grissmer,S
中科院分区：
文献类型：
--
作者：
Chandy,KG;Cahalan,MD;Grissmer,S
使用膜片钳技术结合荧光显微镜，我们发现T细胞中电压门控K+通道表达异常，这是第一个连接小鼠三种不同自身免疫性疾病的分子标志物。来自系统性红斑狼疮、1型糖尿病和实验性过敏性脑脊髓炎的所有已知小鼠模型的CD4−CD8−Thy − 1.2+（双阴性或DN）淋巴细胞与正常小鼠中的表型对应物相比，显示出异常高数量的不寻常K+通道，称为type。来自这些患病小鼠的其他T细胞亚群保持其正常的K+通道表达模式。DN T细胞独特的K+通道表型与自身免疫的发生平行出现。虽然有丝分裂原活化的T细胞和快速增殖的胸腺细胞表现出大量的K+通道，但这些通道属于电生理学上不同的类型，称为n。因此，丰富的1K+通道的表达似乎是与自身免疫相关的DN T细胞的一个有用的标志物，并可能提供一个有价值的工具，描绘DN T细胞在自身免疫性疾病的发病机制中的作用。
Using the patch‐clamp technique in combination with fluorescence microscopy we have found an abnormality in voltage‐gated K+channel expression in T cells that represents the first molecular marker linking three disparate autoimmune diseases in mice. CD4−CD8−Thy‐1.2+(double‐negative or DN) lymphocytes from every known murine model for systemic lupus erythematosus, type‐1 diabetes mellitus and experimental allergic encephalomyelitis exhibit abnormally high numbers of an unusual K+channel, termed typelcompared to their phenotypic counterparts in normal mice. Other T cell subsets from these diseased mice retain their normal pattern of K+channel expression. The unique K+channel phenotype of DN T cells arises in parallel with the onset of autoimmunity. Although mitogen‐activated T cells and rapidly proliferating thymocytes exhibit large numbers of K+channels, these channels are of an electrophysiologically distinct type calledn. Thus, abundant expression of typelK+channels appears to be a useful marker for DN T cells associated with autoimmunity and may provide a valuable tool for delineating the role of DN T cells in the pathogenesis of autoimmune diseases.