Culturable bacterial diversity in hypolithic and peripheral soils in the west of the Hexi Corridor desert and its influencing factors
Culturable bacterial diversity in hypolithic and peripheral soils in the west of the Hexi Corridor desert and its influencing factors
复制标题
河西走廊荒漠西部地下及周边土壤可培养细菌多样性及其影响因素
DOI：
10.3724/sp.j.1226.2020.00047
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发表时间：
2020
影响因子：
1
通讯作者：
Zhang Wei
中科院分区：
文献类型：
--
作者：
He Lifang;Li Shiweng;Zhang Gaosen;Wu Xiukun;Zhang Binglin;Zhang Wei
栖息在沙漠中的微生物对环境变化反应敏感，可能是沙漠生态系统变化的指标。沙漠中的亚底栖微生物群落在土壤形成和有机质积累等生态系统过程中起着至关重要的作用。本研究调查和比较了重土和周边土壤中可培养细菌的群落结构和多样性，以及细菌与环境因子之间的相互作用。用4种不同的培养基分离细菌，并通过16 S rRNA基因序列分析进行鉴定。土壤可培养细菌数量在低层土壤中为3.0× 104 ~ 3.6×10~ 5CFU/g，在周边土壤中为6.5× 104 ~ 5.3×10~ 5CFU/g，周边土壤中细菌数量高于低层土壤。共鉴定出34属98种，其中节杆菌属、芽孢杆菌属和链霉菌属的种类较多，分布较广。可培养细菌群落具有明显的样品特异性，低层土壤中的多样性高于周边土壤。在区域尺度上，可培养菌的分布与环境因子呈规律性变化。在局部范围内，高异质性的hypolithic环境决定了特异性的数量和种类的可培养细菌。
Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem. Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation. This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils, and the interaction between bacteria and environmental factors. The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis. The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×10~4 to 3.6×10~5 CFU/g and from 6.5×10~4 to 5.3×10~5 CFU/g, respectively, indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil. A total of 98 species belonging to 34 genera were identified, among which Arthrobacter, Bacillus, and Streptomyces were found dominantly and widely distributed. The community of culturable bacteria had obvious sample specificity, and the diversity in hypolithic soil was higher than that in peripheral soil. On the regional scale, the distribution of culturable bacteria and the environmental factors showed regular changes. On the local scale, the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.