The new role of LOX-1 in hypertension induced neuronal apoptosis
The new role of LOX-1 in hypertension induced neuronal apoptosis
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LOX-1在高血压诱导的神经细胞凋亡中的新作用
DOI：
10.1016/j.bbrc.2012.07.143
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发表时间：
2012-09-07
影响因子：
3.1
通讯作者：
Niu, Xiaolin
中科院分区：
文献类型：
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作者：
Li, Yali;Duan, Zongming;Niu, Xiaolin
凝集素样氧化低密度脂蛋白（oxLDL）受体-1（LOX-1）最初被鉴定为主要在内皮细胞中表达的oxLDL受体。最近，LOX-1的上调已涉及许多细胞类型中的氧化应激和细胞凋亡。然而，LOX-1在神经元中的表达或LOX-1对神经元凋亡的调节尚未报道。为探讨LOX-1在高血压脑损伤中的作用，我们检测了LOX-1在32周龄SHR和WKY大鼠大脑皮层和海马中的分布，并比较了其表达。免疫荧光显示LOX-1阳性细胞主要分布于感觉信息处理的皮层，主要表达于神经元。SHR组LOX-1、Bax和caspase-3 mRNA表达上调，Bcl-2 mRNA表达下调。与WKY组比较，SHR组LOX-1阳性细胞数和TUNEL阳性细胞数均明显增多。LOX-1与TUNEL阳性细胞共定位，提示LOX-1的表达参与了高血压相关细胞的凋亡。这些结果表明LOX-1在SHR大脑皮层表达上调，其表达与神经元凋亡有关。该事件可能涉及Bax/Bcl-2比值升高。(C)2012 Elsevier Inc. All rights reserved.
Lectin-like oxidized low-density lipoprotein (oxLDL) receptor-1 (LOX-1) was originally identified as a receptor for oxLDL predominantly expressed in endothelial cells. Recently up-regulation of LOX-1 has been implicated in oxidative stress and cell apoptosis in many cell types. However, LOX-1 expression in neurons or regulation of neuronal apoptosis by LOX-1 has not been reported. To investigate the possible roles of LOX-1 in hypertension induced brain damage, we examined the distribution of LOX-1 in cortex and hippocampus and compared its expression in 32-week-old SHR and WKY rats. Immunofluorescence revealed that LOX-1 positive cells were located principally at the cortex involved in sensory information processing and were mainly expressed in neurons. We also found up-regulated mRNA expression of LOX-1, Bax and caspase-3 and down-regulated mRNA expression of Bcl-2 in SHR group. Compared with WKY group, SHR group showed increased LOX-1 positive cells and TUNEL positive cells. Furthermore, double-labeling method indicated that LOX-1 expression was colocalized with TUNEL positive cells, which means that LOX-1 expression was involved in hypertension related cell apoptosis. These findings indicated that LOX-1 expression was up-regulated in the cortex of SHR and its expression has implication in neuronal apoptosis. Elevated Bax/Bcl-2 ratio may be involved under this event. (C) 2012 Elsevier Inc. All rights reserved.