Selection of sequence elements that substitute for the standard AATAAA motif which signals 3' processing and polyadenylation of late simian virus 40 mRNAs.
Selection of sequence elements that substitute for the standard AATAAA motif which signals 3' processing and polyadenylation of late simian virus 40 mRNAs.
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DOI：
10.1093/nar/13.22.8053
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发表时间：
1985-11
影响因子：
14.9
通讯作者：
C. Swimmer;T. Shenk
中科院分区：
文献类型：
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作者：
C. Swimmer;T. Shenk
描述了一种方法，该方法允许选择可以替代参与SV 40晚期mRNA的多腺苷酸化的正常AATAAA六核苷酸的序列。空斑由缺乏基序的病毒DNA产生，迫使获得替代序列。对四种变体进行了表征。所有显示野生型生长动力学和生产正常水平的晚期mRNA和蛋白质。两个变体重新获得了AATAAA元件，一个获得了ATTAAA序列。最后一个变体携带ATTTTTTAAA片段，这表明这种新序列或其某些部分也可以发出多聚腺苷酸添加的信号。
A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants were characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.