STRUCTURE AND EXPRESSION OF ELONGATION FACTOR-1-ALPHA IN TOMATO
STRUCTURE AND EXPRESSION OF ELONGATION FACTOR-1-ALPHA IN TOMATO
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DOI：
10.1093/nar/17.12.4661
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发表时间：
1989-06-26
影响因子：
14.9
通讯作者：
SHEWMAKER, CK
中科院分区：
文献类型：
--
作者：
POKALSKY, AR;HIATT, WR;SHEWMAKER, CK
已从番茄中分离了α-LeEF-1的全长cDNA克隆。延伸因子1亚基（EF-1 α），在蛋白质合成中起主要作用的多肽。由该cDNA编码的448个氨基酸的蛋白质似乎与其它EF-1 α高度同源，与EF 1 α具有高度相似性（75-78%）。先前从低等真核生物和动物中描述的。Southern分析表明EF-1 α.属于番茄中一个4-8个成员的多基因小家族。EF-1 α的表达模式通过北方分析、体外翻译和原位杂交分析了不同番茄组织中的mRNA。EF-1.alpha. mRNA是丰富的种类，并且与在较老的组织中观察到的mRNA水平相比，在发育中的组织如嫩叶和绿色果实中发现更高水平的mRNA。增加的EF-1 α水平。因此，mRNA似乎与发育组织中更高水平的蛋白质合成相关。
A full-length cDNA clone, LeEF-1, has been isolated from tomato for the .alpha. subunit of elongation factor 1 (EF-1.alpha.), a polypeptide which plays a central role in protein synthesis. The 448 amino acid protein encoded by this cDNA appears highly homologous to other EF-1.alpha.s having a high degree of similarity (75-78%) to EF1.alpha. previously described from both lower eukaryotes and animals. Southern analysis indicated that EF-1.alpha. belongs to a small multigene family of 4-8 members in tomato. The pattern of expression of EF-1.alpha. mRNA in various tomato tissues was analyzed by Northern analysis, in vitro translation and in situ hybridization. EF-1.alpha. mRNA is an abundant species and higher levels of mRNA were found in developing tissues such as young leaves and green fruit compared to the mRNA levels observed in older tissues. The increased levels of EF-1.alpha. mRNA therefore appear to correlate with higher levels of protein synthesis in developing tissues.