Inhibition of Bone Marrow Colony Formation by Normal and Leukaemic Human Serum
Inhibition of Bone Marrow Colony Formation by Normal and Leukaemic Human Serum
复制标题
正常和白血病人血清对骨髓集落形成的抑制
DOI：
10.1038/227845a0
复制
发表时间：
1970
期刊：
影响因子：
64.8
通讯作者：
Donald Metcalf
中科院分区：
文献类型：
--
作者：
S. Chan;Donald Metcalf
小鼠和人类的血清和尿液中存在一种因子，它可以刺激琼脂培养物中小鼠骨髓细胞形成粒细胞和巨噬细胞集落1-3。由于 CSF 浓度和集落数量之间存在 S 形关系，因此可以在骨髓培养物中测定这种集落刺激因子 (CSF) 的水平。先前的研究表明，患有各种类型白血病的小鼠的血清脑脊液水平升高1,4。尽管某些白血病患者的血清集落刺激活性似乎很高5，但由于无法检测正常人血清中的CSF，无法直接比较正常人和白血病人血清中的CSF水平。
A FACTOR is present in the serum and urine of mice and humans which can stimulate the formation of granulocytic and macrophage colonies by mouse bone marrow cells in agar culture1–3. Levels of this colony stimulating factor (CSF) can be assayed in bone marrow cultures because of the sigmoid relationship between CSF concentration and colony numbers3. Previous studies showed that serum CSF levels were elevated in mice with various types of leukaemia1,4. Although serum colony stimulating activity seemed to be high in some patients with leukaemia5, a direct comparison of CSF levels in normal and leukaemic human sera was prevented by an inability to detect CSF in normal human serum.