Kinetics of annexin VI, calcium, and phospholipid association and dissociation.
Kinetics of annexin VI, calcium, and phospholipid association and dissociation.
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膜联蛋白 VI、钙和磷脂结合和解离的动力学。
DOI：
10.1021/bi00034a010
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Nelsestuen,GL
中科院分区：
文献类型：
--
作者：
Lu,Y;Bazzi,MD;Nelsestuen,GL
材料和方法材料。卵PC和二棕榈酰-N-丹酰-La-磷脂酰乙醇胺（丹酰-PE）购自Avanti Polar Lipids，Inc。磷脂酸（PA）和磷脂酰乙醇胺（PE）也购自同一公司，并且衍生自eggPC。所有的磷脂都是高纯度的（> 98%，供应商的估计）。聚碳酸酯过滤器（直径100和600 nm）购自NucleoporeCorp。（Costar Co.）其他化学品来自Sigma Chemical Co.，并且是可用的最高等级。通过别处描述的方法从牛脑或肺中纯化膜联蛋白V和膜联蛋白VI（Bazzi & Nelsestuen，1991 b）。囊泡制备。所有磷脂最初储存在氯仿中。混合各组分，并通过氮气流蒸发有机溶剂。然后将样品置于真空下1小时。将干燥的磷脂与Tris缓冲液（50 mM Tris，150 mM NaCl，pH 7.5）混合至终浓度为1.0 mg/mL。通过多次冻融循环并挤出通过孔径为100 nm的聚碳酸酯膜制备大单层囊泡（LUV）（Hope et al.，1985年）。然后在使用前将囊泡用含有100 mM NaCl的Tris缓冲液透析至少4 h。缩略语：PC，磷脂酰胆碱; PE，磷脂酰乙醇胺; PA，磷脂酸;丹酰-PE，二棕榈酰-N-丹酰-磷脂酰乙醇胺; SUV，小单层囊泡; LUV，大单层囊泡; EGTA，乙二醇双（β-氨基乙基醚）-VdV/V/V ′-四乙酸; Tris，三（羟甲基）氨基甲烷; LS，光散射; FET，荧光能量转移。
MATERIALS AND METHODSMaterials. Egg PC and dipalmitoyl-/V-dansyl-La-phosphatidylethanolamine (dansyl-PE) were purchased from Avanti Polar Lipids, Inc. Phosphatidic acid (PA) and phosphatidylethanolamine (PE) were also purchased from the same company and were derived from eggPC. All the phospholipids were of high purity (> 98%, supplier’s esti-mate). Polycarbonate filters (100 and 600 nm in diameter) were purchased from NucleoporeCorp.(Costar Co.). Other chemicals were from Sigma Chemical Co. and were of the highest grade available. Annexin V and annexin VI were purified from bovine brain or lung by methods described elsewhere (Bazzi & Nelsestuen, 1991b). Vesicle Preparation. All the phospholipids were originally stored in chloroform. The components were mixed, and the organic solvent was evaporated by a stream of nitrogen. The sample was then placed under vacuum for 1 h. The dried phospholipids were mixed with Tris buffer (50 mM Tris, 150 mM NaCl, pH 7.5) to a final concentration of 1.0 mg/mL. Large unilamellar vesicles (LUVs) were prepared by multiple freeze—thaw cycles and extrusion through poly-carbonate membranes with a pore size of 100 nm (Hope et al., 1985). The vesicles were then dialyzed at least 4 h against the Trisbuffer containing 100 mM NaCl before being used. Unlessspecified, the results presented were obtained1 Abbreviations: PC, phosphatidylcholine; PE, phosphatidylethanolamine; PA, phosphatidic acid; dansyl-PE, dipalmitoyl-/V-dansyl-Laphosphatidylethanolamine; SUVs, small unilamellar vesicles; LUVs, large unilamellar vesicles; EGTA, ethylene glycol bis (/3-aminoethyl ether)-VdV/V/V'-tetraacetic acid; Tris, tris (hydroxymethyl) aminomethane; LS, light scattering; FET, fluorescence energy transfer.
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以磷脂依赖性方式结合钙的蛋白质。
影响因子：
2.9
作者：
Bazzi,MD;Nelsestuen,GL
通讯作者：
Nelsestuen,GL
酸性磷脂的表面特性：单层和水合液晶与单价和二价金属离子的相互作用。
DOI：
--
发表时间：
1968
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
D. Papahadjopoulos
通讯作者：
D. Papahadjopoulos
影响因子：
3.9
作者：
TAIT, JF;GIBSON, D
通讯作者：
GIBSON, D
膜联蛋白 II 包含两种类型的 Ca(2 ) 结合位点。
影响因子：
4.1
作者：
M. Jost;K. Weber;V. Gerke
通讯作者：
V. Gerke
通过停流光散射进行凝血酶原-膜结合的动力学和机制分析。
影响因子：
2.9
作者：
Wei,GJ;Bloomfield,VA;Resnick,RM;Nelsestuen,GL
通讯作者：
Nelsestuen,GL