Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation
Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation
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DOI：
10.1038/modpathol.3880501
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发表时间：
2002-02-01
期刊：
影响因子：
7.5
通讯作者：
Nagashima, K
中科院分区：
文献类型：
--
作者：
Itoh, T;Orba, Y;Nagashima, K
射频消融（RFA）后，肝细胞癌发生完全坏死和不可逆的持续坏死，其组织学特征为细胞轮廓破坏、同质胞质嗜酸性粒细胞增多和保留的核染色，细胞与活癌细胞明显不同。抗体检测单链DNA（ssDNA）特异性标记的细胞核中正在进行的坏死，但不是活的肿瘤细胞，而人线粒体抗体标记的细胞质中的活细胞，但不是正在进行的坏死细胞。结果表明，RFA导致DNA和蛋白质变性，ssDNA和线粒体蛋白的免疫组化可用于检测RFA后持续坏死，并提供有关该程序有效性的病理信息。
After radiofrequency ablation (RFA), hepatocellular carcinoma undergoes complete necrosis and an ongoing necrosis that is irreversible and characterized histologically by disrupted cell outlines, homogenous cytoplasmic eosinophilia, and preserved nuclear staining, with the cells appearing quite distinct from viable cancer cells. Antibody to detect single-stranded DNA (ssDNA) specifically labeled nuclei in the setting of ongoing necrosis, but not viable tumor cells, whereas human mitochondrial antibody labeled the cytoplasm of viable cells but not cells of ongoing necrosis. The results demonstrate that RFA causes denaturation of both DNA and proteins and that the immunohistochemistry of ssDNA and mitochondrial protein is useful in detection of ongoing necrosis after RFA and provides pathological information on the validity of this procedure.