Studying the mechanism of CD47-SIRPα interactions on red blood cells by single molecule force spectroscopy
Studying the mechanism of CD47-SIRPα interactions on red blood cells by single molecule force spectroscopy
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DOI：
10.1039/c4nr02889a
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发表时间：
2014-01-01
期刊：
影响因子：
6.7
通讯作者：
Wang, Hongda
中科院分区：
文献类型：
--
作者：
Pan, Yangang;Wang, Feng;Wang, Hongda
通过单分子力谱 (SMFS) 在新鲜和实验老化的人红细胞 (hRBC) 上研究了 SIRPa 和 CD47 之间的相互作用力和结合动力学。我们发现CD47在氧化后经历了构象变化，这影响了SIRPa和CD47之间的相互作用力和能垒的位置。我们的结果对于在单分子水平上理解红细胞吞噬机制具有重要意义。
The interaction forces and binding kinetics between SIRPa and CD47 were investigated by single-molecule force spectroscopy (SMFS) on both fresh and experimentally aged human red blood cells (hRBCs). We found that CD47 experienced a conformation change after oxidation, which influenced the interaction force and the position of the energy barrier between SIRPa and CD47. Our results are significant for understanding the mechanism of phagocytosis of red blood cells at the single molecule level.