Surfactant protein D alters allergic lung responses in mice and human subjects
Surfactant protein D alters allergic lung responses in mice and human subjects
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DOI：
10.1016/j.jaci.2008.02.011
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发表时间：
2008-05-01
影响因子：
14.2
通讯作者：
Rothenberg, Marc E.
中科院分区：
文献类型：
--
作者：
Brandt, Eric B.;Mingler, Melissa K.;Rothenberg, Marc E.
背景：表面活性剂蛋白（SP） D已被认为在过敏性气道反应中具有保护作用。目的：探讨SP-D缺乏对小鼠和人气道变态反应的影响。方法：观察SP-D基因缺陷小鼠（sfpd(-/-)）在基线和4次鼻内烟曲霉暴露后的免疫反应。此外，我们还研究了人类SP-D基因中单核苷酸多态性（Met(11)Thr）的意义（已知会降低SP-D功能）。结果：原发性sfpd（-/-）小鼠的巨噬细胞和中性粒细胞支气管肺泡灌洗液水平和大气道粘液产生增加，与肺CCL17水平和CD4(+) T细胞数量增加有关。T(H)2相关抗体水平（IgG1和IgE）在4 ~ 5周龄sfpd（-/-）小鼠中显著降低(P
Background: Surfactant protein (SP) D has been proposed to be protective in allergic airway responses.Objective: We aimed to determine the effect of SP-D deficiency on murine and human airway allergy.Methods: Immunologic responses of SP-D gene-deficient mice (Sftpd(-/-)) at baseline and after 4 intranasal Aspergillus fumigatus exposures were assessed. In addition, the significance of a single nucleotide polymorphism (Met(11)Thr) in the human SP-D gene (known to decrease SP-D function) was investigated. Results: Macrophage and neutrophil bronchoalveolar lavage fluid levels and large airway mucus production were increased in naive Sftpd(-/-) mice in association with increased lung CCL17 levels and CD4(+) T cell numbers. T(H)2-associated antibody levels (IgG1 and IgE) were significantly lower in 4- to 5-week-old Sftpd(-/-) mice (P