Substance P inhibits pancreatic exocrine secretion via a neural mechanism.
Substance P inhibits pancreatic exocrine secretion via a neural mechanism.
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DOI：
10.1152/ajpgi.1999.277.2.g314
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发表时间：
1999-08
期刊：
影响因子：
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通讯作者：
K. Kirkwood;Edward H Kim;X. He;E. Q. Calaustro;Christopher Domush;Shandra K. Yoshimi;E. Grady;J. Maa;N. Bunnett;H. Debas
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文献类型：
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作者：
K. Kirkwood;Edward H Kim;X. He;E. Q. Calaustro;Christopher Domush;Shandra K. Yoshimi;E. Grady;J. Maa;N. Bunnett;H. Debas
本实验观察了感觉神经肽P物质(SP)对血管灌流大鼠胰腺淀粉酶和液体分泌的影响。SP以剂量依赖的方式抑制CCK诱导的淀粉酶释放和促胰液素诱导的胰液流经胰管。灌流液中加入10-10M SP后出现阈值抑制，以10-8M SP抑制最大。神经激肽-1(NK1)和神经激肽-2(NK2)受体拮抗剂均可部分阻断SP的作用。阿托品和河豚毒素可阻断SP诱导的淀粉酶分泌(分别阻断26%和63%)和胰液流量(分别阻断21%和79%)。用辣椒素兴奋胰腺感觉神经(在没有SP的情况下)通过激活NK1受体抑制淀粉酶和胰液流动。我们认为，SP通过间接的神经机制抑制外分泌。
We investigated the effects of the sensory neuropeptide substance P (SP) on amylase and fluid secretion in the isolated vascularly perfused rat pancreas. SP inhibited CCK-induced amylase release and secretin-induced juice flow via the pancreatic duct in a dose-related fashion. Threshold inhibition occurred following addition of 10-10 M SP to the perfusate, and maximal inhibition was seen with 10-8 M SP. The effects of SP were partially blocked by both the neurokinin-1 (NK1) and neurokinin-2 (NK2) receptor antagonists. Atropine and TTX blocked SP-induced effects on both amylase secretion (26 and 63% blockade, respectively) and pancreatic juice flow (21 and 79% blockade, respectively). Excitation of pancreatic sensory nerves using capsaicin (in the absence of SP) inhibited both amylase and pancreatic juice flow via activation of the NK1 receptor. We conclude that SP inhibits exocrine secretion via an indirect neural mechanism.