Long noncoding RNA opa-interacting protein 5 antisense transcript 1 promotes proliferation and invasion through elevating integrin α6 expression by sponging miR-143-3p in cervical cancer
Long noncoding RNA opa-interacting protein 5 antisense transcript 1 promotes proliferation and invasion through elevating integrin α6 expression by sponging miR-143-3p in cervical cancer
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DOI：
10.1002/jcb.27454
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Chen, Caixia
中科院分区：
文献类型：
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作者：
Yang, Jing;Jiang, Bo;Chen, Caixia
越来越多的研究表明，长非编码RNA (lncRNA) 在宫颈癌(CC) 进展中发挥重要作用。然而，lncRNA opa相互作用蛋白5反义转录物1（OIP5-AS1）在CC中的作用和潜在机制仍不清楚。在目前的研究中，我们发现lncRNA OIP5-AS1在CC组织和细胞系中表达上调。 OIP5-AS1的高表达与CC患者的国际妇产科联盟（FIGO）分期晚期、淋巴结转移和较差的总生存率显着相关。通过体外功能测定，我们发现 OIP5-AS1 抑制显着降低 CC 细胞的增殖、集落形成和侵袭能力。此外，我们发现OIP5-AS1可以作为miR-143-3p的竞争性内源RNA来调节ITGA6的表达。救援实验表明，miR-143-3p 抑制剂或 ITGA6 过表达可以逆转 OIP5-AS1 抑制对 CC 细胞增殖和侵袭的抑制作用。此外，OIP5-AS1 抑制可减少体内肿瘤生长。总之，我们证明OIP5-AS1通过海绵miR-143-3p增加ITGA6表达来促进CC细胞的增殖和侵袭，这可能是治疗CC患者的有效治疗靶点。
An increasing number of studies have shown that long noncoding RNAs (lncRNAs) play important roles in cervical cancer (CC) progression. However, the roles and underlying mechanisms of lncRNA opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) involved in the CC remain unclear. In the current study, we found that lncRNA OIP5-AS1 was upregulated in CC tissues and cell lines. High OIP5-AS1 expression was significantly correlated with advanced International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and poor overall survival of patients with CC. Using in vitro function assays, we showed that OIP5-AS1 suppression significantly decreased the proliferation, colony formation, and invasion ability of CC cells. Moreover, we revealed that OIP5-AS1 could act as a competing endogenous RNA of miR-143-3p to regulate the ITGA6 expression. Rescue assays showed that miR-143-3p inhibitors or ITGA6 overexpression could reverse the inhibitory effects of OIP5-AS1 suppression on the proliferation and invasion in CC cells. In addition, OIP5-AS1 suppression reduced tumor growth in vivo. In conclusion, we demonstrated that OIP5-AS1 promoted proliferation and invasion of CC cells via increasing the ITGA6 expression by sponging miR-143-3p, which might be an effective therapeutic target for the treatment of patients with CC.