Principles and applications of ENDOR spectroscopy for structure determination in solution and disordered matrices.
Principles and applications of ENDOR spectroscopy for structure determination in solution and disordered matrices.
复制标题
ENDOR 光谱法用于溶液和无序基质结构测定的原理和应用。
作者：
D. Murphy;R. Farley
电子顺磁共振(EPR)和电子核双共振(Endor)光谱都是描述生物、化学和物理中顺磁系统的非常强大和通用的方法。然而，与EPR相比，Endor仍然是一种应用较少的技术。在本教程中，我们将介绍连续波Endor的基本原理。然后描述了用于冷冻溶液和粉末结构测定的取向选择Endor理论。最后给出了一系列的例子，说明了从Endor谱获得的信息类型。
Both electron paramagnetic resonance (EPR) and electron nuclear double resonance (ENDOR) spectroscopies are extremely powerful and versatile methods for the characterisation of paramagnetic systems in biology, chemistry, and physics. However, by comparison to EPR, ENDOR remains a less widely used technique. In this tutorial review the basic principles of continuous wave ENDOR are described. The theory of orientation selective ENDOR, for structure determination in frozen solutions and powders, is then described. A range of examples, illustrating the type of information obtained from the ENDOR spectrum, is finally presented.