Biochemical properties and pathophysiological roles of cytosolic phospholipase A2s
Biochemical properties and pathophysiological roles of cytosolic phospholipase A2s
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DOI：
10.1016/j.bbalip.2006.08.001
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发表时间：
2006-11-01
影响因子：
4.8
通讯作者：
Shimizu, Takao
中科院分区：
文献类型：
--
作者：
Kita, Yoshihiro;Ohto, Takayo;Shimizu, Takao
磷脂酶A（2）（PLA（2））（EC 3.1.1.4）催化甘油磷脂的sn-2酯键的水解。该酶对于两类脂质介质（脂肪酸代谢物和溶血磷脂相关脂质）的产生以及参与膜磷脂的重塑至关重要。在许多哺乳动物PLA（2）S中，胞质PLA（2）α（cPLA（2）α）通过产生脂质介质在各种生理和病理生理条件下起关键作用。在这里，我们总结了cPLA（2）α的体内意义，从cPLA（2）α缺失小鼠的表型中揭示，以及新发现的cPLA（2）家族酶的特性。我们还简要介绍了定量脂质组学策略，使用液相色谱-质谱，一个强大的工具，全面分析脂质介质。(c)2006 Elsevier B. V.保留所有权利。
Phospholipase A(2) (PLA(2)) (EC 3.1.1.4) catalyzes hydrolysis of the sn-2 ester bond of glycerophospholipids. The enzyme is essential for the production of two classes of lipid mediators, fatty acid metabolites and lysophospholipid-related lipids, as well as being involved in the remodeling of membrane phospholipids. Among many mammalian PLA(2)S, cytosolic PLA(2)alpha (cPLA(2)alpha) plays a critical role in various physiological and pathophysiological conditions through generating lipid mediators. Here, we summarize the in vivo significance of cPLA(2)alpha, revealed from the phenotypes of cPLA(2)alpha-null mice, and properties of newly discovered cPLA(2) family enzymes. We also briefly introduce a quantitative lipidomics strategy using liquid chromatography-mass spectrometry, a powerful tool for the comprehensive analysis of lipid mediators. (c) 2006 Elsevier B.V. All rights reserved.