Uveitis seroreactivity to candidate pANCA antigens: mycobacterial HupB and histone H1(69-171).
Uveitis seroreactivity to candidate pANCA antigens: mycobacterial HupB and histone H1(69-171).
复制标题
葡萄膜炎对候选 pANCA 抗原的血清反应性：分枝杆菌 HupB 和组蛋白 H1(69-171)。
DOI：
10.1080/09273940490912452
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发表时间：
2005
影响因子：
3.3
通讯作者：
Gordon,LynnK
中科院分区：
文献类型：
--
作者：
Kassan,Hallie;Cohavy,Offer;Rosenbaum,JamesT;Braun,Jonathan;Gordon,LynnK
目的部分葡萄膜炎患者表达疾病标志物抗体pANCA。组蛋白H1 （H1）和分枝杆菌HupB （HupB）是最近发现的候选pANCA抗原。本研究提出了H1和HupB是pANCA+葡萄膜炎疾病相关血清反应性靶标的假设。方法对293例葡萄膜炎患者血清进行pANCA活性分类。在ELISA研究中，H1（69-171）和HupB重组蛋白作为抗原靶点。用Western blot分析所选血清的反应性。结果葡萄膜炎患者血清中常检测到ANCA、H1（69 ~ 171）和HupB的活性。ANCA抗体水平与对两种重组抗原的反应性均无显著关系。然而，H1（69-171）和HupB血清活性之间存在显著的正相关。结论H1（69-171）和HupB反应性之间的相关性表明存在一个共享的显性表位，这在相当数量的葡萄膜炎患者中被抗体识别。血清对ANCA和HupB/H1的活性是独立的免疫标志物，可以识别生物学上独特的葡萄膜炎亚群。
PurposeCertain uveitis patients express the disease-marker antibody pANCA. Histone H1 (H1) and mycobacterial HupB (HupB) are recently identified candidate pANCA antigens. This study addresses the hypothesis that H1 and HupB are targets of disease-associated seroreactivity in pANCA+ uveitis.MethodsSera from 293 uveitis patients were categorized for pANCA activity. H1(69-171) and HupB recombinant proteins were used as antigenic targets in ELISA studies. Selected sera were analyzed for reactivity by Western blot.ResultsSeroreactivities to ANCA, H1(69-171), and HupB were frequently detected in uveitis patients. No significant relationship existed between the level of ANCA antibody and reactivity to either recombinant antigen. There was, however, a significant, positive correlation between H1(69-171) and HupB seroreactivities.ConclusionsCorrelation between H1(69-171) and HupB reactivities suggests the presence of a shared dominant epitope, which is recognized by antibodies in a substantial number of uveitis patients. Seroreactivities to ANCA and HupB/H1 are independent immunologic markers that may identify biologically distinctive subsets of uveitis.