A photo-responsive cholesterol capable of inducing a morphological transformation of the liquid-ordered microdomain in lipid bilayers
A photo-responsive cholesterol capable of inducing a morphological transformation of the liquid-ordered microdomain in lipid bilayers
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DOI：
10.1007/s00396-008-1936-5
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发表时间：
2008-10
影响因子：
2.4
通讯作者：
K. Yasuhara;Y. Sasaki;J. Kikuchi
中科院分区：
文献类型：
--
作者：
K. Yasuhara;Y. Sasaki;J. Kikuchi
设计并合成了偶氮苯修饰的胆固醇，用于光诱导脂质双分子层膜结构域转化。在紫外线照射下，胆固醇衍生物通过反式偶氮苯部分的光异构化改变构象。光异构化在液体有序相（Lo）和液体无序相（Ld）中都有效发生。相对比和荧光显微镜观察表明，偶氮苯修饰胆固醇的光异构化诱导了巨型单层囊泡（GUV）的形状转变和Lodomain结构的重组。根据GUV的脂质组成，这种光诱导脂质结构域的转化有两种不同的途径；Lodomain的消失或在Lodomain中出现一个小的Lddomain。
An azobenzene-modified cholesterol was designed and synthesized for photo-induced domain transformation in lipid bilayer membranes. Upon UV-light irradiation, the cholesterol derivative changes the conformation through photoisomerization of the azobenzene moiety fromtrans- tocis-form. The photoisomerization effectively occurred both in liquid-ordered (Lo) and liquid-disordered (Ld) phases. Phase-contrast and fluorescence microscopic observation revealed that photoisomerization of the azobenzene-modified cholesterol induced the shape transformation of giant unilamellar vesicle (GUV) and the reorganization of Lodomain structure. Such a photo-induced transformation of lipid domain gave two different pathways dependent on the lipid composition of GUV; disappearance of the Lodomain or appearance of a small Lddomain with in the Lodomain.