Molecular design and delivery of siRNA
Molecular design and delivery of siRNA
复制标题
DOI：
10.1080/10611860600845397
复制
发表时间：
2006-01-01
影响因子：
4.5
通讯作者：
Taira, Kazunari
中科院分区：
文献类型：
--
作者：
Inoue, Atsushi;Sawata, Shinya Y.;Taira, Kazunari
双链短干扰RNA（siRNA）以序列特异性方式介导基因沉默。由于其特异的基因沉默活性以及最近发现的质粒和病毒驱动的表达，siRNA被广泛应用于研究和治疗。为了在治疗中的应用，进行了努力以优化siRNA表达系统。开发RNA干扰（RNAi）疗法的一个主要障碍是将siRNA递送至靶细胞。新型分子靶向技术的结合，如重组蛋白技术和核糖体展示技术，将使基因沉默剂特异性和有效地递送到靶细胞。
Double stranded short interfering RNAs (siRNAs) mediate gene silencing in a sequence specific manner. By virtue of their specific gene silencing activity and owing to the recent discoveries on their plasmid and virus driven expression, siRNAs are being widely adopted in research and therapeutics. Efforts were made to optimize the siRNA expression system for the application in therapy. One major obstacle in developing RNA interference (RNAi) therapy is the delivery of siRNAs to the target cells. Combination of novel molecular targeting technologies, such as recombinant protein technology and ribosome display technology, will enable to deliver gene silencing agents to target cells specifically and efficiently.