Fast and slow light-induced changes in murine outer retina optical coherence tomography: complementary high spatial resolution functional biomarkers.
Fast and slow light-induced changes in murine outer retina optical coherence tomography: complementary high spatial resolution functional biomarkers.
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小鼠外视网膜光学相干断层扫描的快速和慢速光诱导变化：互补的高空间分辨率功能生物标志物。
DOI：
10.1093/pnasnexus/pgac208
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Qian,Haohua
中科院分区：
文献类型：
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作者：
Gao,Shasha;Zeng,Yong;Li,Yichao;Cohen,EthanD;Berkowitz,BruceA;Qian,Haohua
已经开发了对光刺激的快速（秒）和慢速（分钟至小时）光学相干断层扫描（OCT）响应，以探测具有比经典全视野视网膜电图（ERG）更高空间分辨率的外部视网膜功能。然而，OCT和ERG所揭示的功能信息之间的关系在很大程度上是未知的。在这项研究中，我们直接比较了快速和慢速OCT反应与ERG。快速反应[即视网膜光图（ORG）]由外段（OS）和内段椭圆区（ISez）的反射率变化主导。ORG OS反应比ISez反应具有更快的动力学和更高的光敏感性，并且两者与ERG参数显著不同。西地那非对光转导的抑制降低了ORG的光敏感性，表明ORG反应需要完整的光转导途径。较慢的OCT响应主要由光诱导的视网膜色素上皮（ELM-RPE）厚度和光感受器尖端低反射带（HB）幅度的外界膜变化引起，最大的变化发生在长时间的光刺激后。具有高（129 S6/ev）与低（C57 BL/6 J）ATP（三磷酸腺苷）合成效率的小鼠显示出相似的快速ORG，但不相似的缓慢OCT响应。我们提出，ORG反映被动生理学，如水运动从光感受器，响应于光电流响应（可测量的ERG），而缓慢的OCT响应测量视网膜驱动的生理学在外层视网膜，如黑暗引起的水从视网膜下腔的去除。
Fast (seconds) and slow (minutes to hours) optical coherence tomography (OCT) responses to light stimulation have been developed to probe outer retinal function with higher spatial resolution than the classical full-field electroretinogram (ERG). However, the relationships between functional information revealed by OCT and ERG are largely unexplored. In this study, we directly compared the fast and slow OCT responses with the ERG. Fast responses [i.e. the optoretinogram (ORG)] are dominated by reflectance changes in the outer segment (OS) and the inner segment ellipsoid zone (ISez). The ORG OS response has faster kinetics and a higher light sensitivity than the ISez response, and both differ significantly with ERG parameters. Sildenafil-inhibition of phototransduction reduced the ORG light sensitivity, suggesting a complete phototransduction pathway is needed for ORG responses. Slower OCT responses were dominated by light-induced changes in the external limiting membrane to retinal pigment epithelium (ELM-RPE) thickness and photoreceptor-tip hyporeflective band (HB) magnitudes, with the biggest changes occurring after prolonged light stimulation. Mice with high (129S6/ev) vs. low (C57BL/6 J) ATP(adenosine triphosphate) synthesis efficiency show similar fast ORG, but dissimilar slow OCT responses. We propose that the ORG reflects passive physiology, such as water movement from photoreceptors, in response to the photocurrent response (measurable by ERG), whereas the slow OCT responses measure mitochondria-driven physiology in the outer retina, such as dark-provoked water removal from the subretinal space.