Highly sensitive method for aldehydes detection: Application to furfurals analysis in raisin and bovine milk powder.
Highly sensitive method for aldehydes detection: Application to furfurals analysis in raisin and bovine milk powder.
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DOI：
10.1016/j.aca.2017.08.032
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发表时间：
2017-09
影响因子：
6.2
通讯作者：
Yao Sun;Zhaobing Guan;H. Cai;Yiyong Huang;Yawei Lin;Xiaosong Hu
中科院分区：
文献类型：
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作者：
Yao Sun;Zhaobing Guan;H. Cai;Yiyong Huang;Yawei Lin;Xiaosong Hu
首次将基于硝酮生成的柱前荧光衍生化方法应用于食品中糠醛（F）、5-甲基糠醛（5-MF）和5-羟甲基糠醛（5-HMF）的测定。用N-取代羟胺试剂4-（（羟氨基）丁基）-7-羟基香豆素（HAHC）与糠醛的醛基反应，生成了稳定的硝酮衍生物，并对其荧光性质进行了研究。反应在温和的条件下进行，在30分钟内，高衍生化产率（>93%）。随后在反相柱上在25 min内实现了三种糠醛衍生物的基线分离。检测限为低飞摩尔水平（S/N = 3，每次进样20 μL）。校准曲线的线性范围为0.4-4000 nM，相关系数良好（R2≥ 0.9991）。该方法已应用于牛奶粉、葡萄干等食品样品的分析。回收率在94.7%~ 103.5%之间。该柱前衍生化方法简便、快速、灵敏度高，为醛类化合物在不同基质中的研究提供了一种有效的方法。
A pre-column fluorescent derivatization method based on nitrone formation has been applied to determine furfurals (e.g. furfural (F), 5-methyfurfural (5-MF) and 5-hydroxymethylfurfural (5-HMF)) in food samples for the first time. An N-substituted hydroxylamine reagent 4-((hydroxyamino)butyl)-7-hydroxycoumarin (HAHC) was used to react with the aldehyde group of furfurals to form stable nitrone derivatives with high fluorescence intensities. The reactions proceeded under mild conditions in 30 min with high derivatization yields (>93%). A baseline-separation of three furfurals derivatives was subsequently achieved within 25 min on a reversed-phase column. The detection limits were at the low femtomol level (S/N = 3, 20 μL per injection). The linear range of the calibration curve was 0.4–4000 nM with good correlation coefficients (R2≥ 0.9991). The proposed method was further applied for food sample analysis, such as bovine milk powder and raisin. Satisfactory recoveries were obtained in the range of 94.7%–103.5%. Above all, this pre-column derivatization method is simple, fast and highly sensitive, providing an effective and promising way for future studies of aldehydes in different matrices.