Protein adsorption on titanium surfaces and their effect on osteoblast attachment.
Protein adsorption on titanium surfaces and their effect on osteoblast attachment.
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DOI：
10.1002/jbm.a.10578
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发表时间：
2003-10
期刊：
影响因子：
--
通讯作者：
Y. Yang;R. Cavin;J. Ong
中科院分区：
文献类型：
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作者：
Y. Yang;R. Cavin;J. Ong
本研究的目的是研究白蛋白和纤连蛋白在钛（Ti）表面的吸附以及预吸附的白蛋白和纤连蛋白对体外成骨细胞附着的影响。本研究使用牛血清白蛋白和牛纤连蛋白。孵育 180 分钟后，观察到牛血清白蛋白和纤连蛋白在 Ti 表面上的最大吸附发生。在存在预吸附蛋白质的情况下，观察到与对照钛表面相比，钛表面上的成骨细胞附着增强。然而，细胞附着受到吸附蛋白质类型的影响。观察到预吸附白蛋白对成骨细胞附着量没有显着影响。与对照Ti表面和预吸附白蛋白的Ti表面相比，观察到预吸附纤连蛋白15分钟的Ti表面显着增加成骨细胞附着，而预吸附纤连蛋白180分钟的Ti表面不影响细胞附着。此外，蛋白质预吸附钛表面上附着细胞的细胞形态不受本研究中使用的蛋白质类型的影响。从该研究得出的结论是，与白蛋白相比，吸附在钛表面上的纤连蛋白的浓度更高。此外，还得出结论，钛表面纤连蛋白的浓度在控制细胞附着方面发挥着作用。
The objective of this study was to investigate the adsorption of albumin and fibronectin on titanium (Ti) surfaces and the effect of preadsorbed albumin and fibronectin on osteoblast attachment in vitro. Bovine serum albumin and bovine fibronectin were used in this study. Maximum adsorption of bovine serum albumin and fibronectin on Ti surfaces was observed to occur after 180-min incubation. In the presence of preadsorbed proteins, osteoblast attachment on Ti surfaces was observed to be enhanced compared to control Ti surfaces. However, cell attachment was affected by the types of protein adsorbed. Preadsorbed albumin was observed to have no significant effect on the amount of osteoblast cells attached. In comparison to control Ti surface and Ti surfaces preadsorbed with albumin, Ti surfaces preadsorbed with fibronectin for 15 min was observed to significantly increase osteoblast cell attachment, whereas Ti surfaces preadsorbed with fibronectin for 180 min did not affect cell attachment. In addition, cell morphology of the attached cells on protein preadsorbed Ti surfaces was not affected by the type of protein used in this study. It was concluded from this study that the concentration of fibronectin adsorbed on Ti surfaces was higher compared to albumin. In addition, it was also concluded that the concentration of fibronectin on Ti surfaces plays a role in governing cell attachment.