Orthodontic mechanical tension effects on the myofibroblast expression of alpha-smooth muscle actin
Orthodontic mechanical tension effects on the myofibroblast expression of alpha-smooth muscle actin
复制标题
正畸机械张力对肌成纤维细胞α-平滑肌肌动蛋白表达的影响
DOI：
10.2319/101609-578.1
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发表时间：
2010-09-01
影响因子：
3.4
通讯作者：
Jing, Yan
中科院分区：
文献类型：
--
作者：
Meng, Yao;Han, Xianglong;Jing, Yan
目的：检测体内和体外正畸牙移动过程中张力侧肌成纤维细胞的形成以及张力诱导的肌成纤维细胞α-平滑肌肌动蛋白（α-SMA）的表达。材料与方法：本体内研究采用50只6周龄雄性大鼠，右上颌第一磨牙近中移动作为实验组，左上颌第一磨牙作为对照。在力加载后第0、3、5、7和14天处死大鼠。通过免疫组织化学检查用 a-SMA 鉴定的肌成纤维细胞。为了进行体外研究，获得了人牙周膜（PDL）成纤维细胞。对成纤维细胞施加循环机械张力0、1、3、6和12小时。采用透射电子显微镜检测肌成纤维细胞的超微结构。通过实时定量PCR对α-SMA mRNA基因表达进行定量。免疫荧光检测α-SMA的表达，Western blotting定量α-SMA的表达。结果：在体内，实验组和对照组均鉴定出表达α-SMA的肌成纤维细胞。实验组张力区α-SMA的表达随着时间的推移逐渐增加，并在第14天达到最大值。在体外，透射电镜可发现细胞内的纤维连接和肌动蛋白微丝。与对照组相比，循环机械张力在12小时可显着诱导α-SMA表达（P < .01）。结论：PDL中存在肌成纤维细胞。肌成纤维细胞中α-SMA的表达在体内和体外在张力下均显着上调。 （角度正交。2010；80：912-918。）
Objective: To detect myofibroblast formation on the tension side during orthodontic tooth movement in vivo and myofibroblast expression of alpha-smooth muscle actin (alpha-SMA) induced by tension both in vivo and in vitro.Materials and Methods: Fifty 6-week male rats were used in this in vivo study, and the right maxillary first molar was moved mesially, which served as the experimental group, and the left maxillary first molar served as the control. Rats were sacrificed at days 0, 3, 5, 7, and 14 after force loading. Myofibroblasts, identified with a-SMA, were examined through immunohistochemistry. For the in vitro study, human periodontal ligament (PDL) fibroblasts were obtained. Cyclic mechanical tension was applied to the fibroblasts for 0, 1, 3, 6, and 12 hours. Transmission electron microscopy was used to detect the ultrastructure of myofibroblasts. alpha-SMA mRNA gene expression was quantified by real-time quantitative PCR. The expression of alpha-SMA was detected by immunofluorescence and quantified by Western blotting.Results: In vivo, the myofibroblasts expressing alpha-SMA were identified both in the experimental group and in the control group. The expressions of alpha-SMA were increased in the tension areas of the experimental group over time, and reached the maximum in day 14. In vitro, fibronexus junctions and actin microfilaments in the cells could be found with transmission electron microscopy. Cyclic mechanical tension could significantly induce alpha-SMA expression at 12 hours (P < .01) than the controls.Conclusions: Myofibroblasts existed in the PDL. The expressions of alpha-SMA in the myofibroblasts were significantly up regulated under tension both in vivo and in vitro. (Angle Orthod. 2010;80:912-918.)