A human TRIM5α B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus
A human TRIM5α B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus
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DOI：
10.1016/j.virol.2008.05.008
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发表时间：
2008-09-01
期刊：
影响因子：
3.7
通讯作者：
Sodroski, Joseph
中科院分区：
文献类型：
--
作者：
Diaz-Griffero, Felipe;Perron, Michel;Sodroski, Joseph
人TRIM 5 α限制N-嗜性鼠白血病病毒（N-MLV）感染，但不限制B-嗜性MLV（B-MLV）感染。在这里，我们研究了人TRIM 5 α的B30.2/SPRY结构域突变体，这些突变体在逆转录之前获得抑制B-MLV感染的能力，而不丧失限制N-MLV感染的能力。值得注意的是，这些突变体获得了减少感染细胞胞质溶胶中颗粒B-MIV衣壳量的能力。此外，这些突变体获得了限制SIVmac和HIV-2感染的能力。B-MLV和SIVmac感染在逆转录之前被突变体TRIM 5 α蛋白阻断。因此，B30.2/SPRY结构域的改变可以增加受人TRIM 5a限制的逆转录病毒的范围，这也导致限制性逆转录病毒衣壳的过早脱壳的能力。(C)2008年爱思唯尔公司All rights reserved.
Human TRIM5 alpha restricts N-tropic murine leukemia virus (N-MLV) but not B-tropic MLV (B-MLV) infection. Here we study B30.2/SPRY domain mutants of human TRIM5 alpha that acquire the ability to inhibit B-MLV infection prior to reverse transcription Without losing the ability to restrict N-MLV infection. Remarkably, these Mutants gain the ability to decrease the amount of particulate B-MIV capsids in the cytosol of infected cells. In addition, these mutants gain the ability to restrict SIVmac and HIV-2 infection. B-MLV and SIVmac infections were blocked by the mutant TRIM5 alpha proteins prior to reverse transcription. Thus, the range of retroviruses restricted by human TRIM5a can be increased by changes in the B30.2/SPRY domain, which also result in the ability to cause premature uncoating of the restricted retroviral capsid. (C) 2008 Elsevier Inc. All rights reserved.