Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken
Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken
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DOI：
10.5713/ajas.2006.1065
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发表时间：
2006-05
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影响因子：
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通讯作者：
J. Oh;H. Kong;Jun-Heon Lee;I. Choi;S. J. Lee;Sang-Goo Lee;B. Sang;C. Choi;B. Cho;G. Jeon;Hye-Ja Lee
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作者：
J. Oh;H. Kong;Jun-Heon Lee;I. Choi;S. J. Lee;Sang-Goo Lee;B. Sang;C. Choi;B. Cho;G. Jeon;Hye-Ja Lee
鸟类解偶联蛋白（avUCP）是线粒体转运蛋白超家族的成员，其将质子进入线粒体基质与ATP合成解偶联。采用序列分析方法对韩国本地鸡（KNC）avUCP基因的核苷酸多态性进行了鉴定。这项研究确定了10个单核苷酸多态性（SNPs）的avUCP基因。我们分析了avUCP基因的SNPs，以调查该基因的多态性是否可能导致KNC经济性状的数量变异。经济性状的3个显著多态位点是avUCP C+282 T（平均体重，p<0.05）、avUCP C+433 T（日增重，p<0.05）和avUCP T+1316 C（日增重，p<0.05）。各SNP的频率分别为0.125（avUCP基因外显子1区C+282 T）、0.150（avUCP基因内含子1区C+433 T）和0.15（avUCP基因外显子3区T+1316 C）。在所鉴定的SNPs中，一对SNPs（基因型CC，C+282T和TT，avUCP C+433T）显示出最高的日产蛋率（p<0.05）和平均体重（p<0.05），频率为0.067。对avUCP基因的研究为该基因的遗传研究和KNC经济性状的选择提供了依据。
The avian uncoupling protein (avUCP) is a member of the mitochondrial transporter superfamily that uncouples proton entry in the mitochondrial matrix from ATP synthesis. The sequencing analysis method was used to identify nucleotide polymorphisms within the avUCP gene in Korean native chicken (KNC). This study identified ten single nucleotide polymorphisms (SNPs) in the avUCP gene. We analyzed the SNPs of the avUCP gene to investigate whether polymorphism in the gene might be responsible for quantitative variations in economic traits in KNC. Three significant polymorphic sites for economic traits were avUCP C+282T (mean body weight, p<0.05), avUCP C+433T (daily percent lay, p<0.05), and avUCP T+1316C (daily percent lay, p<0.05). The frequency of each SNPwas 0.125 (C+282T in avUCP gene exon 1 region), 0.150 (C+433T in avUCP gene intron 1 region), and 0.15 (T+1316C in avUCP gene exon 3 region), respectively. Among the identified SNPs, one pair of SNPs (genotype CC, C+282T and TT, avUCP C+433T) showed the highest daily percent lay (p<0.05) and mean body weight (p<0.05) and the frequency was 0.067. This study of the avUCP gene could be useful for genetic studies of this gene and selection on economic traits for KNC.