Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens
Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens
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DOI：
10.1111/tpj.13764
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Zhenbing Zheng;Shan Gao;Li Huan;Guangce Wang
中科院分区：
文献类型：
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作者：
Zhenbing Zheng;Shan Gao;Li Huan;Guangce Wang
由于其高效的同源重组能力和独特的进化地位，小立苔藓在遗传和进化研究中开始受到越来越多的关注。原丝体是配子体的丝状阶段，在分离原生质体中具有重要意义。此外，原丝体在基因工程中也有广泛的应用。然而，原丝体的诱导和状态维持的困难限制了其更广泛的应用。在本工作中，原丝体在稀释的海水介质中被有效地诱导，并且在没有进一步细胞分化的情况下保持了丝状状态。转移到淡水培养基后，原丝体的发育过程恢复，进入芽组装和配子体形成。此外，转录组分析表明，当原丝体在稀释的海水介质中生长时，植物激素信号转导途径下调。与转录组结果一致的是，原丝体不能对稀释液中添加的吲哚-3-乙酸和6-苯氨基嘌呤作出反应。根据这些结果，我们得出结论：稀释的海水培养基会阻碍原丝体的分化。这一结果可能为未来原丝体的生产提供新的见解。
Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte, is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to freshwater medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were downregulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to the addition of indole-3-acetic acid and 6-benzylaminopurine to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium blocks the differentiation of protonema. This result could provide a novel insight to benefit future protonema production.