Calbindin-D28K cells selectively contact intra-SCN neurons
Calbindin-D28K cells selectively contact intra-SCN neurons
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DOI：
10.1016/s0306-4522(01)00604-2
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Silver, R
中科院分区：
文献类型：
--
作者：
Lesauter, J;Kriegsfeld, LJ;Silver, R
钙结合蛋白-盘免疫反应细胞紧密包装，在仓鼠的视交叉上核的尾侧的一个离散区域内。这些细胞接收直接视网膜输入，并对光脉冲作出反应，呈Fos阳性。了解它们的传入和传出联系对于理解视交叉上核的组织结构是必要的。本研究的第一个目的是确定钙结合蛋白和其他肽能细胞的视交叉上核之间的相互联系，使用epi和共聚焦显微镜和内视交叉上核束追踪，结果表明，基本上所有的钙结合蛋白细胞接收大量的appositions从血管活性肠多肽（VIP）。神经肽Y和5-羟色胺纤维，并且大多数接受来自胃泌素释放肽（GRP）和胆囊收缩素（CCK）纤维的沉积。从VIP、GRP和CCK细胞中可以看到相互连接，但令人惊讶的是。而不是来自背内侧加压素细胞。将生物素化的葡聚糖胺注射到视交叉上核中表明，视交叉上核腹外侧投射到整个核。而视交叉上核的背侧和内侧区则密集地投射到除calbindin区以外的大部分核。结果表明，91%的P物质细胞、42%的GRP细胞和60%的VIP细胞共表达Calbindin-D-28 K，提示视交叉上核细胞间存在高度特化的局部连接。(C)2002年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
Calbindin-Disk-immunoreactive cells are tightly packed,within a discrete region of the caudal aspect of the suprachiasmatic nuclei of hamsters. These cells receive direct retinal input and are Fos-positive in response to a light pulse. Knowledge of their afferent and efferent connections is necessary to understand suprachiasmatic nucleus organization. The first aim of the present study is to identify interconnections between calbindin and other peptidergic cells of the suprachiasmatic nuclei, using epi- and confocal microscopy and intra-suprachiasmatic nucleus tract tracing, The results indicate that essentially all calbindin cells receive numerous appositions from vasoactive intestinal polypeptide (VIP). neuropeptide Y and serotonin fibers and that most receive appositions from gastrin releasing peptide (GRP) and cholecystokinin (CCK) fibers. Reciprocal connections are seen from VIP, GRP and CCK cells but surprisingly. not from dorsomedial vasopressin cells, Injection of biotinylated dextran amine into the suprachiasmatic nucleus indicates that the ventrolateral suprachiasmatic nucleus projects to the entire nucleus. while the dorsal and medial regions of the suprachiasmatic nucleus project densely to most of the nucleus, except to the calbindin region. Analysis of colocalization of the peptides in the calbindin cell region shows that 91% of the substance P cells, 42% of the GRP cells and 60% of the VIP cells in the calbindin subnucleus coexpress calbindin-D-28K.Our results reveal a highly specialized topographical organization of connections among suprachiasmatic nucleus cells. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.