Basal lamina components are concentrated in premuscle masses and at early acetylcholine receptor clusters in chick embryo hindlimb muscles.
Basal lamina components are concentrated in premuscle masses and at early acetylcholine receptor clusters in chick embryo hindlimb muscles.
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基底层成分集中在鸡胚后肢肌肉的前肌块和早期乙酰胆碱受体簇中。
DOI：
10.1016/0012-1606(88)90343-0
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发表时间：
1988
影响因子：
2.7
通讯作者：
Yorde,DE
中科院分区：
文献类型：
--
作者：
Godfrey,EW;Siebenlist,RE;Wallskog,PA;Walters,LM;Bolender,DL;Yorde,DE
作为表征体内肌细胞分化和神经支配过程中基底层成分功能的第一步，我们通过免疫组织化学方法确定了鸡胚后肢肌肉发育过程中三种成分的表达模式：层粘连蛋白、集聚蛋白（一种乙酰胆碱受体（AChR）聚集蛋白）相关蛋白和硫酸乙酰肝素蛋白聚糖。使用抗集聚蛋白的单克隆抗体从鱼雷中纯化蛋白质，并表征来自胚胎和成年鸡的集聚蛋白样蛋白。在早期后肢芽（第 19 阶段）中，针对层粘连蛋白和集聚蛋白的抗体对外胚层基底膜进行染色，并以普遍的点状分布与肢体间充质结合。然而，随着背侧和腹侧前肌块的浓缩（第 22-23 阶段），层粘连蛋白和集聚蛋白样蛋白（而非蛋白多糖）的间充质免疫反应性集中在这些生肌区域。值得注意的是，这些分子优先在肢体的生肌区域积聚，随后 1-2 天出现肌肉特异性蛋白、成肌细胞融合和肌肉神经支配。从我们第一次在第 26 阶段的新形成的肌管上观察到它们时，所有三个基底层成分都优先与所有 AChR 簇相关。这些抗原在肢体神经支配之前外植的肢体间质三维胶原凝胶培养物中的定位，与胚胎肢体发育过程中看到的染色模式平行。这些结果表明肢体间充质固有的基底层分子是肌发生和突触分化的早期标志物，并表明这些成分在肌发生和突触发生的初始阶段发挥重要作用。
As an initial step in characterizing the function of basal lamina components during muscle cell differentiation and innervationin vivo, we have determined immunohistochemically the pattern of expression of three components—laminin, proteins related to agrin (an acetylcholine receptor (AChR)-aggregating protein), and a heparan sulfate proteoglycan—during the development of chick embryo hindlimb muscles. Monoclonal antibodies against agrin were used to purify the protein from theTorpedoray and to characterize agrin-like proteins from embryonic and adult chicken. In early hindlimb buds (stage 19), antibodies against laminin and agrin stained the ectodermal basement membrane and bound to limb mesenchyme with a generalized, punctate distribution. However, as dorsal and ventral premuscle masses condensed (stage 22–23), mesenchymal immunoreactivity for laminin and agrin-like proteins, but not the proteoglycan, became concentrated in these myogenic regions. Significantly, the preferential accumulation of these molecules in myogenic regions of the limb preceded by 1–2 days the appearance of muscle-specific proteins, myoblast fusion, and muscle innervation. All three basal lamina components were preferentially associated with all AChR clusters from the time we first observed them on newly formed myotubes at stage 26. Localization of these antigens in three-dimensional collagen gel cultures of limb mesenchyme, explanted prior to innervation of the limb, paralleled the staining patterns seen during limb development in the embryo. These results indicate that basal lamina molecules intrinsic to limb mesenchyme are early markers for myogenic and synaptic differentiation, and suggest that these components play important roles during the initial phases of myogenesis and synaptogenesis.