Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates
Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates
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DOI：
10.1111/j.1439-0507.2009.01757.x
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发表时间：
2011-01-01
期刊：
影响因子：
4.9
通讯作者：
Neves, Rejane P.
中科院分区：
文献类型：
--
作者：
Lima-Neto, Reginaldo G.;Beltrao, Eduardo I. C.;Neves, Rejane P.
许多研究报道了白色念珠菌对上皮细胞的粘附，但对近平滑念珠菌的粘附及其在宿主细胞表面识别中的作用知之甚少。本研究旨在评估20 ℃的依从性与20 ℃的依从性之间的相关性。白色念珠菌12株。用凝集素组织化学方法检测近平滑菌株对人颊上皮细胞的作用及真菌细胞表面糖的表达。通过在酵母悬浮液（107细胞ml-1）中孵育上皮细胞进行粘附测定，并将过氧化物酶缀合的凝集素（Con A、WGA、UEA I和PNA，25 μ g ml-1）用于凝集素组织化学。结果表明，C. albicans比C.近平滑（P < 0.01），并且个体菌株差异与高含量的细胞表面α-l-岩藻糖残基相关，如UEA I染色模式所示。基于所用凝集素的糖特异性，这些结果表明，细胞表面糖缀合物上的L-岩藻糖残基可能代表所研究的酵母菌株与宿主之间相互作用的识别分子（r = 0.6985，P = 0.0045）。此外，我们的研究结果还表明真菌细胞壁中存在α-d-葡萄糖/α-d-甘露糖、N-乙酰-d-葡萄糖胺/N-乙酰神经氨酸和d-半乳糖/N-乙酰-d-半乳糖胺。
P>Many studies have described the adherence of Candida albicans to epithelial cells but little is known about Candida parapsilosis adhesion and its role in host cell surface recognition. This study was designed to evaluate the correlation between the adherence of 20 C. albicans and 12 C. parapsilosis strains to human buccal epithelial cells and the expression of fungal cell surface carbohydrates using lectin histochemistry. Adherence assays were carried out by incubating epithelial cells in yeast suspensions (107 cells ml-1) and peroxidase conjugated lectins (Con A, WGA, UEA I and PNA at 25 mu g ml-1) were used for lectin histochemistry. The results showed that adherence was overall greater for C. albicans than for C. parapsilosis (P < 0.01) and that the individual strain differences correlated with a high content of cell surface alpha-l-fucose residues as indicated by the UEA I staining pattern. Based on the saccharide specificity of the lectins used, these results suggest that l-fucose residues on cell surface glycoconjugates may represent recognition molecules for interactions between the yeast strain studied and the host (r = 0.6985, P = 0.0045). In addition, our results indicated the presence of alpha-d-glucose/alpha-d-mannose, N-acetyl-d-glucosamine/N-acetylneuraminic acid and d-galactose/N-acetyl-d-galactosamine in fungal cell wall.