Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses
Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses
复制标题
非生物胁迫下玉米 (Zea mays L.) 生长素响应 GH3 家族基因的全基因组鉴定和表达分析。
DOI：
10.1111/jipb.12327
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发表时间：
2015-09-01
影响因子：
11.4
通讯作者：
Shen, Chenjia
中科院分区：
文献类型：
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作者：
Feng, Shangguo;Yue, Runqing;Shen, Chenjia
生长素通过调节生长素应答家族基因的表达参与植物生长发育的各个方面。GH 3（GretchenHagen 3）基因是植物生长素应答基因的三大家族之一，它通过催化生长素结合和结合游离吲哚乙酸（Indoline-3-Acetate，IAA）与氨基酸的结合来参与植物生长素的体内平衡。然而，GH 3基因如何在玉米中响应非生物胁迫和各种激素的功能在很大程度上是未知的。在这里，最新更新的玉米（Zea mays L.）参考基因组序列用于表征和分析来自玉米的ZmGH 3家族基因。结果表明，13个ZmGH 3基因被定位在5条玉米染色体上（共10条染色体）。高度多样化的基因结构和组织特异性表达模式表明这些基因在响应环境胁迫和激素刺激时功能多样化的可能性。ZmGH 3基因的表达模式响应于几种非生物胁迫（盐，干旱和镉）和主要的应力相关激素（脱落酸，水杨酸和茉莉酸）。各种环境因素抑制生长素自由IAA含量在玉米根表明，这些非生物胁迫和激素可能会改变GH 3介导的生长素水平。ZmGH 3基因对各种非生物胁迫和胁迫相关激素的响应表明，ZmGH 3参与玉米对环境胁迫的耐受性。
Auxin is involved in different aspects of plant growth and development by regulating the expression of auxin-responsive family genes. As one of the three major auxin-responsive families, GH3 (Gretchen Hagen3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free indole-3-acetic acid (IAA) to amino acids. However, how GH3 genes function in responses to abiotic stresses and various hormones in maize is largely unknown. Here, the latest updated maize (Zea mays L.) reference genome sequence was used to characterize and analyze the ZmGH3 family genes from maize. The results showed that 13 ZmGH3 genes were mapped on five maize chromosomes (total 10 chromosomes). Highly diversified gene structures and tissue-specific expression patterns suggested the possibility of function diversification for these genes in response to environmental stresses and hormone stimuli. The expression patterns of ZmGH3 genes are responsive to several abiotic stresses (salt, drought and cadmium) and major stress-related hormones (abscisic acid, salicylic acid and jasmonic acid). Various environmental factors suppress auxin free IAA contents in maize roots suggesting that these abiotic stresses and hormones might alter GH3-mediated auxin levels. The responsiveness of ZmGH3 genes to a wide range of abiotic stresses and stress-related hormones suggested that ZmGH3s are involved in maize tolerance to environmental stresses.