A mouse model of intracranial aneurysm: technical considerations.
A mouse model of intracranial aneurysm: technical considerations.
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DOI：
10.1007/978-3-7091-0693-8_6
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hashimoto, Tomoki
中科院分区：
文献类型：
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作者：
Tada, Yoshiteru;Kanematsu, Yasuhisa;Kanematsu, Miyuki;Nuki, Yoshitsugu;Liang, Elena I;Wada, Kosuke;Makino, Hiroshi;Hashimoto, Tomoki
将弹性蛋白酶单次立体定位注射到高血压小鼠右侧基底池的脑脊液中可诱发颅内动脉瘤。该小鼠模型在 3-4 周内产生大动脉瘤结构，其发生率为 60-80%。该模型中的颅内动脉瘤概括了人类颅内动脉瘤的关键病理特征。有几个技术因素对于该模型中成功诱导颅内动脉瘤至关重要。将针尖精确地立体定位到脑脊液空间中尤为重要。该模型中的动脉瘤形成可以作为未来研究的简单且易于解释的结果，利用各种抑制剂、基因敲除小鼠或转基因小鼠来测试特定分子和通路在颅内动脉瘤病理生理学中的作用。
Intracranial aneurysms can be induced by a single stereotaxic injection of elastase into the cerebrospinal fluid at the right basal cistern in hypertensive mice. This mouse model produces large aneurysm formations with an incidence of 60–80% within 3–4 weeks. Intracranial aneurysms in this model recapitulate key pathological features of human intracranial aneurysms. Several technical factors are critical for the successful induction of intracranial aneurysm in this model. Precise stereotaxic placement of the needle tip into the cerebrospinal fluid space is especially important. Aneurysm formations in this model can serve as a simple and easily interpretable outcome for future studies that utilize various inhibitors, knockout mice, or transgenic mice to test roles of specific molecules and pathways in the pathophysiology of intracranial aneurysms.