Thermostable cellulase production of Aspergillus fumigatus Z5 under solid-state fermentation and its application in degradation of agricultural wastes
Thermostable cellulase production of Aspergillus fumigatus Z5 under solid-state fermentation and its application in degradation of agricultural wastes
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DOI：
10.1016/j.ibiod.2011.04.005
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发表时间：
2011-08-01
影响因子：
4.8
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
--
作者：
Liu, Dongyang;Zhang, Ruifu;Shen, Qirong
分离到一株木质纤维分解菌Z5，经鉴定为烟曲霉属，以木质纤维原料为底物进行固态发酵(SSF)，对其产纤维素酶的能力进行了研究。对烟曲霉Z5产纤维素酶的培养条件进行了优化，结果表明，对于羧甲基纤维素酶和滤纸酶，最适培养温度为50℃，初始含水率为80%，初始接种量为7%，CMC酶活力为526.3 U g(-1)，滤纸酶活力为144.6 U g(-1)干重。粗酶的最适温度为50℃，最适pH为5.0。酶谱分析表明，当培养物中含有纤维素类物质时，烟曲霉Z5可分泌8种CMC酶。将该菌株分泌的粗酶进一步用于玉米秸秆的预处理，并将酶解液作为酿酒酵母生产乙醇的底物。生物乙醇得率为0.112 g g(-1)干基质，是一种很有前途的生物乙醇生产菌种。(C)2011爱思唯尔有限公司。保留所有权利。
A lignocellulosic decomposing fungus Z5 was isolated and identified as Aspergillus fumigatus, its capacity to produce cellulase was assessed under solid-state fermentation (SSF) using lignocellulosic materials as substrates. Cultivation conditions of A. fumigatus Z5 for cellulase production were optimized, results showed that for carboxymethyl cellulase (CMCase) and filter paper enzyme (FPase), the best condition was 50 degrees C, 80% initial moisture, initial pH 4.0 and 7% initial inoculum, the average activity of CMCase activity, FPase activity reached 526.3 and 144.6 U g(-1) dry weight (dw) respectively, much higher than most of previous reports of this genus. Optimal temperature and pH for the CMCase activity of the crude enzyme were found to be 50 degrees C and 5.0, respectively. Zymogram analysis showed that eight kinds of CMCase were secreted by A. fumigatus Z5 when cellulose-containing materials were supplied in the culture. The crude enzyme secreted by the strain was further applied to hydrolyze pretreated corn stover and the enzymatic hydrolysate was used as substrate for ethanol production by Saccharomyces cerevisiae. The yield of bio-ethanol was 0.112 g g(-1) dry substrate (gDS), suggesting that it is a promising fungus in the bio-ethanol production process. (C) 2011 Elsevier Ltd. All rights reserved.