Role of evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) in the antibacterial immunity of Marsupenaeus japonicus
Role of evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) in the antibacterial immunity of Marsupenaeus japonicus
复制标题
Toll 通路中进化保守的信号中间体 (ECSIT) 在日本对虾抗菌免疫中的作用
DOI：
10.1016/j.dci.2014.04.016
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发表时间：
2014-10-01
影响因子：
2.9
通讯作者：
Kang, Cui-Jie
中科院分区：
文献类型：
--
作者：
Ding, Ding;Chen, Xiao-Wei;Kang, Cui-Jie
Toll/Toll样受体（TLR）信号通路在动物先天免疫中具有重要作用。Toll通路中的进化保守信号传导中间体（ECSIT）是作为Toll/TLR和骨形态发生蛋白信号传导通路的衔接蛋白发挥功能的蛋白质。ECSIT也是哺乳动物巨噬细胞杀菌活性的关键组分。然而，ECSIT在甲壳类动物中的功能仍不清楚。在这项研究中，我们克隆和鉴定了一个功能性ECSIT同源物，MjEC-SIT 1，从库鲁马虾Marsupenaeuseums。MjEcsit 1的互补DNA长1442个碱基对，具有1221个碱基对的开放阅读框，编码407个残基的多肽。在血细胞、鳃、肝胰腺、胃、心脏、肠、睾丸和卵巢中检测到MjEcsit 1的转录物。这种转录物被革兰氏阳性细菌（金黄色葡萄球菌）和革兰氏阴性细菌（鳗弧菌）注射上调。通过双链RNA注射敲低MjEcsit 1增加了M.我是S.金黄色葡萄球菌的挑战，并削弱细菌清除能力的M。体内培养。此外，抑制MjEcsit 1可抑制S.金黄色葡萄球菌注射液结果表明MjECSIT 1在M. - 是的(C)2014爱思唯尔有限公司版权所有。
The Toll/Toll-like receptor (TLR) signaling pathway has an important role in the innate immunity of animals. Evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) is a protein that functions as an adaptor protein for the Toll/TLR and bone morphogenetic protein signaling pathways. ECSIT is also a key component in the macrophage bactericidal activity of mammals. However, the function of ECSIT in crustaceans remains unclear. In this study, we cloned and identified a functional ECSIT homologue, MjEC-SIT 1, from kuruma shrimp Marsupenaeus japonicus. The complementary DNA of MjEcsit 1 is 1442 base pairs long, with an open reading frame of 1221 base pairs that encodes a 407-residue polypeptide. Transcripts of MjEcsit 1 are detected in hemocytes, gills, hepatopancreas, stomach, heart, intestines, testes, and ovaries. Such transcripts are upregulated by Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Vibrio anguillarum) injections. The knockdown of MjEcsit 1 by double-stranded RNA injection increases the sensitivity of M. japonicus to S. aureus challenge and weakens the bacterial clearance ability of M. japonicus in vivo. In addition, suppressing MjEcsit 1 restrains the upregulation of two anti-lipopolysaccharide factors by S. aureus injection. The results indicate that MjECSIT 1 is important in the antibacterial immunity of M. japonicus. (C) 2014 Elsevier Ltd. All rights reserved.