A closed eye contact lens model of corneal inflammation. Part 1: Increased synthesis of cytochrome P450 arachidonic acid metabolites.
A closed eye contact lens model of corneal inflammation. Part 1: Increased synthesis of cytochrome P450 arachidonic acid metabolites.
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发表时间：
1995-04
影响因子：
4.4
通讯作者：
M. Conners;R. Stoltz;S. Webb;J. Rosenberg;M. Dunn;N. Abraham;M. Laniado‐Schwartzman
中科院分区：
文献类型：
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作者：
M. Conners;R. Stoltz;S. Webb;J. Rosenberg;M. Dunn;N. Abraham;M. Laniado‐Schwartzman
目的从炎症参数和角膜上皮细胞匀浆对花生四烯酸代谢的角度，研究隐形眼镜诱导的闭合眼角膜炎症模型。方法将58%水的etafilcon A水凝胶人工晶状体植入兔眼，每只眼2个晶状体，然后用丝线缝合约90%的巩膜。通过裂隙灯生物显微镜、主观炎症评分、角膜测厚和角膜上皮[1-(14)C]-花生四烯酸代谢来分析前表面在9天内的炎症事件。结果水凝胶隐形眼镜戴镜后前表面炎症反应呈进行性变化(r=0.999)。角膜中央厚度进行性增加，并与炎症评分呈正相关(r=0.995)。[1-(14)C]-花生四烯酸在角膜上皮细胞匀浆中的代谢导致两种主要产物12-羟基二十碳四烯酸(12-HETE)和12-羟基二十碳三烯酸(12-HETrE)的形成。在9天以上，12-羟色胺和12-羟色胺的合成、主观炎症评分(r=0.963)和角膜厚度的进行性增加(r=0.971)之间存在相关性。结论在这种配戴模式下，角膜上皮细胞合成二十烷基硫酸酯的能力呈时间依赖性增加，12-HETE和12-HETrE的产生与原位炎症反应密切相关。12-HETE和12-HETrE的合成与前表面炎症程度之间的关系表明，这些二十烷基类化合物等可能是闭合眼佩戴水凝胶隐形眼镜引起的炎症反应的介质。
PURPOSE To characterize a model of contact lens-induced corneal inflammation in the closed eye, with respect to inflammatory parameters and the metabolism of arachidonic acid by homogenates of the corneal epithelium. METHODS Rabbit eyes were fitted with extended wear etafilcon A (58% water) hydrogel contact lenses in stacked fashion (two lenses per eye), followed by a silk suture tarsorrhaphy of approximately 90%. The anterior surface was analyzed over a 9-day period for inflammatory events through slit lamp biomicroscopy, subjective inflammatory scoring, corneal pachymetry, and corneal epithelial [1-(14)C]-arachidonic acid metabolism. RESULTS Hydrogel contact lens wear in the closed eye resulted in a progressive anterior surface inflammatory response correlated over time (r = 0.999). Central corneal thickness progressively increased and was also correlated to the inflammatory score (r = 0.995). [1-(14)C]-arachidonic acid metabolism by homogenates of the corneal epithelium resulted in the time-dependent formation of two major products, 12-hydroxyeicosatetraenoic acid (12-HETE) and 12-hydroxyeicosatrienoic acid (12-HETrE). Correlations were established between the synthesis of 12-HETE and 12-HETrE, the subjective inflammatory score (r = 0.963) and the progressive increase in corneal thickness (r = 0.971), over 9 days. CONCLUSIONS With this model of contact lens wear, eicosanoid synthesizing capacity of the corneal epithelium showed a time-dependent increase in the production of 12-HETE and 12-HETrE strongly correlating to the in situ inflammatory response. The relationship between 12-HETE and 12-HETrE synthesis and the degree of anterior surface inflammation implicate these eicosanoids, among others, as mediators of the inflammatory response to hydrogel contact lens wear in the closed eye.