liguleless1 encodes a nuclear-localized protein required for induction of ligules and auricles during maize leaf organogenesis
liguleless1 encodes a nuclear-localized protein required for induction of ligules and auricles during maize leaf organogenesis
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DOI：
10.1101/gad.11.5.616
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发表时间：
1997-03-01
影响因子：
10.5
通讯作者：
Freeling, M
中科院分区：
文献类型：
--
作者：
Moreno, MA;Harper, LC;Freeling, M
玉米舌叶和玉米耳廓是玉米叶片上发育在叶鞘和叶片交界处的结构。在缺乏无舌1 （Ig1）基因表达的情况下，舌和耳廓不形成，叶鞘边界不发育为鞘和叶片之间的精确线。利用活化因子（Activator, Ac）转座元件作为分子标记，分离并克隆了Ig1等位基因Ig1-m1。对体细胞相关部门的分析证实了LG1基因产物以细胞自主的方式起作用。cDNA克隆和对LG1 mRNA的RT-PCR分析表明，Ig1基因在发育中的玉米叶片原基舌状区表达水平非常低，可能早在质体期6或更早。在异源系统中的细胞定位研究表明，LG1产物只定位于细胞核。预测的LG1蛋白的氨基酸序列在很大程度上是新的，但包含一个77个氨基酸的内部结构域，与最近在Antirhinum majus中发现的两个SQUAMOSA启动子结合蛋白1和2 （SBP1和SBP2）中的结构域非常相似。
The maize ligule and auricle are structures on the maize leaf that develop at the boundary of the sheath and blade. In the absence of liguleless1 (Ig1) gene expression, ligule and auricle are not formed, and the blade-sheath boundary does not develop as an exact line between sheath and blade. By using the Activator (Ac) transposable element as a molecular tag, a novel Ig1 allele, Ig1-m1, was isolated and cloned. Analysis of somatic revertant sectors confirmed that the LG1 gene product functions in a cell-autonomous fashion. cDNA cloning as well as RT-PCR analysis of the LG1 mRNA indicate that the Ig1 gene is expressed at very low levels in the ligular region of developing maize leaf primordia, perhaps as early as plastochron 6 or earlier. Cellular localization studies in a heterologous system indicate that the LG1 product localizes exclusively to the nucleus. The predicted amino acid sequence of the LG1 protein is largely novel but contains an internal domain of 77 amino acids with significant similarity to a domain present in two recently identified SQUAMOSA PROMOTER-BINDING proteins 1 and 2 (SBP1 and SBP2) in Antirhinum majus.