Role of metabolic spatiotemporal dynamics in regulating biofilm colony expansion
Role of metabolic spatiotemporal dynamics in regulating biofilm colony expansion
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DOI：
10.1073/pnas.1706920115
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发表时间：
2018-04-17
影响因子：
11.1
通讯作者：
Onuchic, Jose N.
中科院分区：
文献类型：
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作者：
Bocci, Federico;Suzuki, Yoko;Onuchic, Jose N.
细胞命运决定通常由生物网络调节，但越来越多的证据表明，细胞间通讯和环境压力在细胞群体的行为中起着至关重要的作用。最近的一项微流控实验表明，两个细胞群体的代谢相互依赖在枯草杆菌生物膜的扩张过程中产生了集体振荡动态。我们开发了一个建模框架的相关的代谢电路在一个殖民地中的细胞的时空动态。我们阐明了代谢物扩散的作用和需要两个不同的细胞群体观察振荡。独特的是，这种描述捕获的发病和此后稳定的振荡动力学膨胀过程中，并预测在各种环境条件下的阻尼振荡的存在。该建模方案提供了深入了解细胞如何整合来自外部信号和细胞间通信的信息，以确定最佳生存策略和/或最大化多细胞系统中的细胞适应性。
Cell fate determination is typically regulated by biological networks, yet increasing evidences suggest that cell-cell communication and environmental stresses play crucial roles in the behavior of a cell population. A recent microfluidic experiment showed that the metabolic codependence of two cell populations generates a collective oscillatory dynamic during the expansion of a Bacillus subtilis biofilm. We develop a modeling framework for the spatio-temporal dynamics of the associated metabolic circuit for cells in a colony. We elucidate the role of metabolite diffusion and the need of two distinct cell populations to observe oscillations. Uniquely, this description captures the onset and thereafter stable oscillatory dynamics during expansion and predicts the existence of damping oscillations under various environmental conditions. This modeling scheme provides insights to understand how cells integrate the information from external signaling and cell-cell communication to determine the optimal survival strategy and/or maximize cell fitness in a multicellular system.