Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis.
Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis.
复制标题
DOI：
10.1101/gad.292235.116
复制
发表时间：
2017-01-15
影响因子：
10.5
通讯作者：
Bevan MW
中科院分区：
文献类型：
--
作者：
Dong H;Dumenil J;Lu FH;Na L;Vanhaeren H;Naumann C;Klecker M;Prior R;Smith C;McKenzie N;Saalbach G;Chen L;Xia T;Gonzalez N;Seguela M;Inze D;Dissmeyer N;Li Y;Bevan MW
在这项研究中，董等人。研究了协调植物器官特征形状和大小的分子机制。作者描述了一种名为DA1的泛素激活的多肽酶，它通过协调调节蛋白的失稳来调节植物器官形成过程中细胞增殖的持续时间以及向内复制和分化的转变。器官的特征形状和大小是由细胞增殖模式和最终细胞大小确定的，但协调这些模式的潜在分子机制尚不清楚。在这里，我们描述了一种名为DA1的泛素激活的多肽酶，它限制了拟南芥器官生长过程中细胞增殖的持续时间。肽酶被两个E3环连接酶激活，Big Brother(BB)和DA2随后被激活的多肽酶切割并破坏稳定性。在BB的情况下，切割通过N端规则途径的环E3连接酶蛋白分解1(PRT1)导致不稳定。DA1多肽酶活性还可以裂解促进细胞增殖的脱泛素酶UBP15，以及促进细胞增殖和抑制内复制的转录因子teosinte BRANCED 1/CycloidEA/PCF 15(TCP15)和TCP22。我们认为，DA1多肽酶活性通过协调调节蛋白的失稳来调节植物器官形成过程中细胞增殖的持续时间以及向内复制和分化的转变。
In this study, Dong et al. investigated the molecular mechanisms coordinating characteristic shapes and sizes of organs in plants. The authors characterize a ubiquitin-activated peptidase called DA1, which regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins. The characteristic shapes and sizes of organs are established by cell proliferation patterns and final cell sizes, but the underlying molecular mechanisms coordinating these are poorly understood. Here we characterize a ubiquitin-activated peptidase called DA1 that limits the duration of cell proliferation during organ growth in Arabidopsis thaliana. The peptidase is activated by two RING E3 ligases, Big Brother (BB) and DA2, which are subsequently cleaved by the activated peptidase and destabilized. In the case of BB, cleavage leads to destabilization by the RING E3 ligase PROTEOLYSIS 1 (PRT1) of the N-end rule pathway. DA1 peptidase activity also cleaves the deubiquitylase UBP15, which promotes cell proliferation, and the transcription factors TEOSINTE BRANCED 1/CYCLOIDEA/PCF 15 (TCP15) and TCP22, which promote cell proliferation and repress endoreduplication. We propose that DA1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins.