Characterization of PC12 cell proliferation and differentiation-stimulated by ECM adhesion proteins and neurotrophic factors
Characterization of PC12 cell proliferation and differentiation-stimulated by ECM adhesion proteins and neurotrophic factors
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DOI：
10.1023/a:1026363018805
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发表时间：
2003-11-01
影响因子：
3.7
通讯作者：
Desai, TA
中科院分区：
文献类型：
--
作者：
Attiah, DG;Kopher, RA;Desai, TA
在体内影响轴突生长的各种因素中，有一种是活跃生长的神经纤维与它们在分化过程中遇到的各种基质的物理化学相互作用。一些实验已经探索了细胞外基质（ECM）在控制神经元分化中的作用。然而，在再生和发育过程中神经元和ECM组分之间相互作用的性质在很大程度上是一个推测的问题。虽然以前的研究已经探讨了一些ECM粘附蛋白和神经营养因子对神经突生长的影响，没有进行系统的方法，允许同时比较不同的表面条件与不同的神经营养因子。出于建立允许合理设计稳定的神经元/生物材料界面的受控环境的必要性，评估了NGF和FGF-2对铺在胶原和层粘连蛋白修饰的表面上的PC 12细胞的行为的长期影响。从大鼠肾上腺髓质移植的嗜铬细胞瘤细胞系PC_（12）细胞，作为研究神经元分化机制的模型，在7-22天的实验中，通过定性和定量分析细胞计数、神经突数目、神经突平均长度、和神经突稳定性。神经突稳定性的测定是根据神经营养因子（NGF/FGF-2）撤出后对损失的抵抗力。目前的研究结果表明，ECM粘附蛋白胶原和层粘连蛋白在促进PC 12增殖方面同样有效。还注意到，在神经突数量、平均长度和稳定性方面，补充NGF的胶原蛋白培养物在为PC 12分化提供长期支持方面显著更有效。(C)2003 Kluwer Academic Publishers.
Among the various elements which influence axonal outgrowth in vivo is the physicochemical interaction of actively outgrowing nerve fibers with the various substrata they encounter during differentiation. Several experiments have explored the role of the extracellular matrix (ECM) in the control of neuronal differentiation. The nature, however, of the interactions between neurons and components of the ECM during regeneration and development are largely a matter of speculation. Although previous studies have already explored the influence of a number of ECM adhesion proteins and neurotrophic factors on neurite outgrowth, none have been carried in a systematic approach that allows for the simultaneous comparison of different surface conditions in relation to different neurotrophic factors. Motivated by the necessity of establishing controlled environments that allow for the rational design of stable neuronal/biomaterial interfaces, the long-term effects of NGF and FGF-2 on the behavior of PC12 cells plated on collagen and laminin modified surfaces were evaluated. A pheochromocytoma cell line derived from transplantable rat adrenal medulla, PC12 cells have been commonly employed as an instructive model for studying the underlying mechanisms of neuronal differentiation.Long-term characterization of PC12 proliferation and neuronal differentiation for an experimental duration of 7-22 days was achieved by both qualitatively and quantitatively assaying for cell count, neurite number, neurite mean length, and neurite stability. Neurite stability was determined in terms of resistance to loss after neurotrophic factor (NGF/FGF-2) withdrawal. The present findings demonstrate that ECM adhesion proteins collagen and laminin are equally effective in promoting PC12 proliferation. It was noted as well that NGF supplemented collagen cultures are significantly more efficient in providing long-term support to PC12 differentiation in terms of neurite number, mean length, and stability. (C) 2003 Kluwer Academic Publishers.