Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR
Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR
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DOI：
10.1016/s0168-1605(02)00481-6
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发表时间：
2003-07-25
影响因子：
5.4
通讯作者：
Bhagwat, AA
中科院分区：
文献类型：
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作者：
Bhagwat, AA
使用人工污染的新鲜农产品设计并评估了能够同时检测大肠杆菌 O157:H7、单核细胞增生李斯特菌和沙门氏菌菌株的方案。官方分析化学家协会 (AOAC) 批准的三种人类病原体的聚合酶链反应 (PCR) 检测方法经过修改，可实现高通量的同时实时检测。该方法包括 PCR 产物的熔解曲线分析，作为验证性测试。当新鲜农产品用含有大肠杆菌 O157:H7 和鼠伤寒沙门氏菌的人工污染水清洗至 1 至 10 个细胞/ml 的预测水平以及单核细胞增生李斯特氏菌 1000 个细胞/ml 的预测水平时，修改后的方案成功检测到了所有三种病原体。同时监测多种病原体的能力将节省时间并提高我们确保食品安全的能力。 (C) 2002 Elsevier Science B.V. 保留所有权利。
A protocol enabling simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains was devised and evaluated using artificially contaminated fresh produce. Association of Official Analytical Chemists (AOAC)-approved polymerase chain reaction (PCR) detection methods for three human pathogens were modified to enable simultaneous and real-time detection with high throughput capability. The method includes a melting-curve analysis of PCR products, which serves as confirmatory test. The modified protocol successfully detected all three pathogens when fresh produce was washed with artificially contaminated water containing E. coli O157:H7 and S. typhimurium down to the predicted level of 1 to 10 cells/ml and L. monocytogenes at 1000 cells/ml. The ability to monitor several pathogens simultaneously will save time and increase our ability to assure food safety. (C) 2002 Elsevier Science B.V. All rights reserved.