Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage
Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage
复制标题
DOI：
10.1038/ncomms4890
复制
发表时间：
2014-05-01
影响因子：
16.6
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Hao, Chenhui;Li, Xiang;Mao, Chengde
RNA nanotechnology promises rational design of RNA nanostructures with wide array of structural diversities and functionalities. Such nanostructures could be used in applications such as small interfering RNA delivery and organization of in vivo chemical reactions. Though having impressive development in recent years, RNA nanotechnology is still quite limited and its programmability and complexity could not rival the degree of its closely related cousin: DNA nanotechnology. Novel strategies are needed for programmed RNA self-assembly. Here, we have assembled RNA nanocages by re-engineering a natural, biological RNA motif: the packaging RNA of phi29 bacteriophage. The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering.
