MOSQUITO INFECTIVITY IS DIRECTLY RELATED TO THE PROPORTION OF REPETITIVE DNA IN PLASMODIUM-BERGHEI
MOSQUITO INFECTIVITY IS DIRECTLY RELATED TO THE PROPORTION OF REPETITIVE DNA IN PLASMODIUM-BERGHEI
复制标题
DOI：
10.1016/0166-6851(82)90048-2
复制
发表时间：
1982-01-01
影响因子：
1.5
通讯作者：
ZENOBI, P
中科院分区：
文献类型：
--
作者：
BIRAGO, C;BUCCI, A;ZENOBI, P
在最后一个完整的周期后，通过注射器在小鼠中传播了一株伯氏肺孢子虫(NK65)超过120代，并对以下参数进行了监测：感染蚊子的能力，诱导卵囊形成；外周血中存在形态可识别的配子体；从红细胞内种群提取的DNA中是否存在重复成分。目前的结果证实了重复DNA比例与对蚊子的传染性之间的相关性，相关系数r=.**图形**。在注射器传播过程中，观察到这两个量平行下降。由NK 65菌株在注射器传播过程中的不同时间衍生的有限数量的克隆株在克隆时具有亲本菌株的感染性，从而证实在感染阶段，来自滋殖子循环的单个无性寄生虫能够起源整个周期。分化为活跃的配子体需要扩增基因组的一部分。
A strain of P. berghei (NK 65) was followed during syringe transmission in mice for over 120 passages after the last complete cycle, while the following parameters were monitored: capacity to infect mosquitoes, inducing oocyst formation; presence in the peripheral blood of morphologically identifiable gametocytes; presence of a repetitive component in the DNA extracted from intraerythrocytic population. Present results confirm the correlation between proportion of repetitive DNA and infectivity towards mosquitoes with a correlation coefficient r = .**GRAPHIC**. A parallel decrease of the 2 quantities is observed in the course of syringe transmission. A limited number of cloned lines, derived from strain NK 65 at different times during syringe transmission, shared the infectivity properties of the parent strain at the moment of cloning, thus confirming that in the infective stage single asexual parasites from the shizogonic cycle are able to originate the whole cyle. Differentiation into active gametocytes involves amplification of a portion the genome.